Identification of a tRNA-specific nuclear export receptor
Identification of a tRNA-specific nuclear export receptor
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DOI：
10.1016/s1097-2765(00)80036-2
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发表时间：
1998-02-01
期刊：
影响因子：
16
通讯作者：
Gorlich, D
中科院分区：
文献类型：
--
作者：
Kutay, U;Lipowsky, G;Gorlich, D
在真核生物中，tRNA在细胞核中合成，经过几个成熟步骤后输出到细胞质。在这里，我们确定exportin-t作为tRNA输出的特定介质。它是一种RanGTP结合、输入素β相关因子，主要位于核内。它在细胞核和细胞质之间快速穿梭，并与核孔复合体相互作用。Exportin-t以高亲和力直接结合tRNA。其细胞浓度在非洲爪蟾卵母细胞被认为是出口的所有tRNA测试的限速，判断显微注射实验。RanGTP调节底物-exportin-t相互作用，使得tRNA可以优先结合在细胞核中并在细胞质中释放。
In eukaryotes, tRNAs are synthesized in the nucleus and after several maturation steps exported to the cytoplasm. Here, we identify exportin-t as a specific mediator of tRNA export. It is a RanGTP-binding, importin beta-related factor with predominantly nuclear localization. It shuttles rapidly between nucleus and cytoplasm and interacts with nuclear pore complexes. Exportin-t binds tRNA directly and with high affinity. Its cellular concentration in Xenopus oocytes was found to be rate-limiting for export of all tRNAs tested, as judged by microinjection experiments. RanGTP regulates the substrate-exportin-t interaction such that tRNA can be preferentially bound in the nucleus and released in the cytoplasm.