THE CONSTRUCTION OF BACILLUS-THURINGIENSIS STRAINS EXPRESSING NOVEL ENTOMOCIDAL DELTA-ENDOTOXIN COMBINATIONS
THE CONSTRUCTION OF BACILLUS-THURINGIENSIS STRAINS EXPRESSING NOVEL ENTOMOCIDAL DELTA-ENDOTOXIN COMBINATIONS
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DOI：
10.1042/bj2700133
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发表时间：
1990-08-15
影响因子：
4.1
通讯作者：
ELLAR, DJ
中科院分区：
文献类型：
--
作者：
CRICKMORE, N;NICHOLLS, C;ELLAR, DJ
使用我们最近报道的电穿孔方法来转化苏云金芽孢杆菌 [Bone 和 Ellar (1989) FEMS Microbiol。莱特。 [58, 171-178]，克隆的苏云金芽孢杆菌杀虫δ-内毒素基因已被引入几种天然苏云金芽孢杆菌菌株中。在许多情况下，产生的转化体表达其天然毒素和克隆毒素，产生具有更广泛毒性谱的菌株。 var 的介绍。将拟步虫毒素基因导入苏云金芽孢杆菌变种。 israelensis 产生了具有抗菜粉蝶（菜粉蝶）活性的菌株，而亲本菌株均不具备这种活性。我们进一步讨论了协同作用的可能性以及通过这种方法引入克隆 DNA 的相关问题。
Using our recently reported method of electroporation to transform Bacillus thuringiensis [Bone and Ellar (1989) FEMS Microbiol. Lett. 58, 171-178], cloned B. thuringiensis entomocidal .delta.-endotoxin genes have been introduced into several native B. thuringiensis strains. In many cases the resulting transformants expressed both their native toxins and the cloned toxin, producing strains with broader toxicity spectra. The introduction of the var. tenebrionis toxin gene into B. thuringiensis var. israelensis resulted in a strain with activity against Pieris brassicae (cabbage white butterfly), an activity which neither parent strain possesses. We discuss further the possibility of synergism and also the problems associated with introducing cloned DNA by this method.