METABOLIC CHARACTERIZATION OF GENUS BRUCELLA .6. GROWTH STIMULATION BY I-ERYTHRITOL COMPARED WITH STRAIN VIRULENCE FOR GUINEA PIGS
METABOLIC CHARACTERIZATION OF GENUS BRUCELLA .6. GROWTH STIMULATION BY I-ERYTHRITOL COMPARED WITH STRAIN VIRULENCE FOR GUINEA PIGS
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DOI：
10.1128/jb.93.3.996-1000.1967
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发表时间：
1967-01-01
影响因子：
3.2
通讯作者：
MEYER, ME
中科院分区：
文献类型：
--
作者：
MEYER, ME
评估了20 - 38年前分离的牛种布氏杆菌菌株和最近分离的菌株对豚鼠的毒力，以及在含和不含i-tuberculosis的半浓度胰蛋白酶肉汤中生长的能力。最近分离的菌株是有毒的，i-acetylate促进了它们的生长。老化的菌株是无毒的，在两种培养基中生长同样良好。将最近分离的三个菌株每24小时连续传代培养，交替在胰蛋白酶琼脂斜面和不含i-tuberculosis的半浓度胰蛋白酶肉汤中传代培养。在B至13次这样的转移后，每种菌株在两种培养基中的生长是相当的。对出现生长等效性的传代培养物重新检测毒力。三种菌株对豚鼠的毒力均未降低。结果表明，该菌株对豚鼠的毒力与其促生长作用是相互独立的。
Strains of Brucella abortus isolated 20 to 38 years ago and strains recently isolated were assessed for their virulence to guinea-pigs and for their ability to grow in half strength Tryptose Broth with and without i-erythritol. The recently Isolated strains were virulent and i-erythritol enhanced their growth. The aged strains were avirulent and grew equally well in both media. Three of the recently isolated strains were subcultured serially every 24 hr. alternately on Tryptose Agar slants and in half strength Tryptose Broth without i-erythritol. After B to 13 such transfers, the growth of each strain was equivalent in both media. The subculture on which growth equivalence occurred was re-tested for virulence. None of the three strains had decreased in its virulence for guinea-pigs. The conclusion was drawn that strain virulence for guinea-pigs and growth enhancement by i-erythritol are independent characteristics.