Studies on Rats with Islet Beta Cell Tumors Induced by Nicotinamide and Streptozotocin
Studies on Rats with Islet Beta Cell Tumors Induced by Nicotinamide and Streptozotocin
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烟酰胺和链脲佐菌素诱导大鼠胰岛β细胞瘤的研究
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发表时间：
1976
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影响因子：
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通讯作者：
Bauer Ge
中科院分区：
文献类型：
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作者：
P. K. Dixit;Bauer Ge
通过烟酰胺和链脲佐菌素联合治疗诱导大鼠胰岛β细胞腺瘤。治疗后三周，在随后表现出大β细胞肿瘤的动物中观察到葡萄糖耐量的显著变化。治疗8个月后，根据明显的低血糖症已知患有β细胞肿瘤的大鼠，后来通过尸检证实，对葡萄糖负荷的反应不同。一些荷瘤大鼠表现出对葡萄糖负荷的快速反应，它们的血糖水平适度升高，并迅速恢复到正常或低于正常水平;这些动物被描述为具有“快速作用的肿瘤”。患有“慢作用肿瘤”的大鼠对葡萄糖负荷反应迟钝;它们的血糖模式与亚糖尿病动物相似。具有β细胞肿瘤的动物在葡萄糖施用后30分钟表现出升高的血清胰岛素水平。β细胞腺瘤的胰岛素生物合成通过[14 C]亮氨酸体外掺入胰岛素原和胰岛素来证明。在研究的少数肿瘤样本中，观察到葡萄糖对胰岛素生物合成的刺激作用。感谢William E博士。杜林，厄普约翰公司，感谢密歇根州卡拉马祖市的Steven Koziol先生赠送链脲佐菌素，感谢新泽西皮斯卡特维市Pharmacia Lab的Steven Koziol先生赠送Phadebas胰岛素检测试剂盒。明尼苏达州医学基金会资助了这项研究。谨以此文纪念已故的阿诺德·拉扎罗博士。
Summary Islet beta cell adenomata were induced in rats by combined treatment with nicotinamide and streptozotocin. Three weeks after treatment marked alterations in glucose tolerance were noted in animals which later exhibited large beta cell tumors. Eight months after treatment, the rats known to have beta cell tumors on the basis of marked hypoglycemia and later confirmed by autopsy showed variable response to a glucose load. Some tumor-bearing rats showed fast response to glucose load, their blood sugar levels were elevated moderately and returned to normal or below normal levels rapidly; these animals are described as having “fast-acting tumors.” Rats with “slow-acting tumors” responded sluggishly to a glucose load; their blood glucose pattern was similar to that of subdiabetic animals. Animals with beta cell tumors exhibited elevated serum insulin levels 30 min after glucose administration. Insulin biosynthesis by beta cell adenomata was demonstrated by in vitro incorporation of [14C]leucine into proinsulin and insulin. In the small number of tumor samples studied, a stimulatory effect of glucose on insulin biosynthesis was observed. Our grateful thanks to Dr. William E. Dulin, The Upjohn Co., Kalamazoo, Michigan, for the gift of streptozotocin, and to Mr. Steven Koziol of Pharmacia Lab, Piscataway, New Jersey, for Phadebas Insulin Test Kits. The Minnesota Medical Foundation aided this research. This paper is dedicated to the memory of the late Dr. Arnold Lazarow.