DIFFERENTIAL INFECTION OF RECEPTOR-MODIFIED HOST-CELLS BY RECEPTOR-SPECIFIC INFLUENZA-VIRUSES
DIFFERENTIAL INFECTION OF RECEPTOR-MODIFIED HOST-CELLS BY RECEPTOR-SPECIFIC INFLUENZA-VIRUSES
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DOI：
10.1016/0168-1702(85)90006-1
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发表时间：
1985-01-01
期刊：
影响因子：
5
通讯作者：
PAULSON, JC
中科院分区：
文献类型：
--
作者：
CARROLL, SM;PAULSON, JC
已经检查了具有对比受体特异性的流感病毒感染含有确定序列的唾液酸寡糖受体决定簇的受体修饰的MDCK细胞的能力。用唾液酸酶处理细胞以除去唾液酸并使其对感染具有抗性，然后用唾液酸转移酶和CMP-唾液酸孵育以恢复SA α 2，6 Gal或SA α 2，3Gal键中的唾液酸。先前显示分别优先结合SA α 2，6 Gal和SA α 2，3Gal键的病毒A/RI/5+/57和A/duck/Ukraine/1/63，发现其表现出与受体特异性雅阁的受体修饰细胞的差异感染。用两种病毒的混合物共感染SA α 2，3Gal衍生的细胞导致SA α 2，3Gal特异性A/duck/Ukraine/1/63病毒的选择性增殖。结果表明，细胞表面受体介导选择流感病毒的受体特异性变体的潜力。
Influenza viruses of contrasting receptor specificity have been examined for their ability to infect receptor-modified MDCK cells containing sialyloligosaccharide receptor determinants of defined sequence. Cells were treated with sialidase to remove sialic acid and render then resistant to infection and were then incubated with sialyltransferase and CMP-sialic acid to restore sialic acid in the SA.alpha.2,6Gal or SA.alpha.2,3Gal linkages. The viruses A/RI/5+/57 and A/duck/Ukraine/1/63, previously shown to exhibit preferential binding of SA.alpha.2,6Gal and SA.alpha.2,3Gal linkages, respectively were found to exhibit differential infection of the receptor-modified cells in accord with their receptor specificity. Coinfection of SA.alpha.2,3Gal derivatized cells with a mixture of the two viruses resulted in selective propagation of the SA.alpha.2,3Gal-specific A/duck/Ukraine/1/63 virus. The results demonstrate the potential for cell surface receptors to mediate selection of receptor-specific variants of influenza virus.