Tandem repeat distribution of gene transcripts in three plant families.
Tandem repeat distribution of gene transcripts in three plant families.
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DOI：
10.1590/s1415-47572009005000091
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发表时间：
2009-10
影响因子：
2.1
通讯作者：
de Oliveira AC
中科院分区：
文献类型：
--
作者：
da Maia LC;de Souza VQ;Kopp MM;de Carvalho FI;de Oliveira AC
串联重复序列（微卫星或SSRs）是植物遗传研究中极具潜力的分子标记。现代策略包括将这些标记在广泛研究的物种和孤儿物种之间转移。计算机分析允许研究微卫星的分布模式，并预测哪些基序更容易在物种间转移。对3科10种植物的微卫星和小卫星的转录序列（单基因）进行了调查。不同物种的串联重复发生率不同，在1.47% ~ 11.28%之间。在植物科内部和科间发现了相似和不同的模式。结果还表明，基因组大小和串联重复序列在表达区域之间缺乏关联。讨论了物种间基序的保守性及其对基因组进化和动力学的意义。
Tandem repeats (microsatellites or SSRs) are molecular markers with great potential for plant genetic studies. Modern strategies include the transfer of these markers among widely studied and orphan species. In silico analyses allow for studying distribution patterns of microsatellites and predicting which motifs would be more amenable to interspecies transfer. Transcribed sequences (Unigene) from ten species of three plant families were surveyed for the occurrence of micro and minisatellites. Transcripts from different species displayed different rates of tandem repeat occurrence, ranging from 1.47% to 11.28%. Both similar and different patterns were found within and among plant families. The results also indicate a lack of association between genome size and tandem repeat fractions in expressed regions. The conservation of motifs among species and its implication on genome evolution and dynamics are discussed.