Pre-binding dynamic range and sensitivity enhancement for immuno-sensors using nanofluidic preconcentrator
Pre-binding dynamic range and sensitivity enhancement for immuno-sensors using nanofluidic preconcentrator
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DOI：
10.1039/b717220f
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发表时间：
2008-01-01
期刊：
影响因子：
6.1
通讯作者：
Han, Jongyoon
中科院分区：
文献类型：
--
作者：
Wang, Ying-Chih;Han, Jongyoon
几乎所有的免疫生物传感器本质上都受到可用于靶分子的抗体的质量的限制，并且获得针对给定靶分子的高灵敏度抗体是一个挑战。我们描述了一种高效和灵活的方法，以提高免疫检测灵敏度和结合动力学，使用纳米流体为基础的电动预浓缩器。该装置是基于电荷的生物分子浓缩器和基于珠的免疫测定的微流体集成。由于预浓缩器可以将局部生物分子浓度增加许多数量级，因此它为免疫传感器提供了更好的灵敏度和更快的结合动力学。通过30分钟的预浓缩，我们能够将免疫测定的灵敏度（分子背景）从50 pM以上提高到100 fM以下。此外，通过调整预浓缩时间，我们可以切换给定的基于微珠的测定的检测范围（从1010 000 ng ml（-1）到0.01-10000 ng ml（-1）），以具有更宽的动态检测范围。由于该系统可以提高检测灵敏度和动态范围，因此可以用于解决常见疾病生物标志物检测中最关键的检测问题。
Almost all immuno-biosensors are inherently limited by the quality of antibodies available for the target molecule, and obtaining a highly sensitive antibody for a given target molecule is a challenge. We describe a highly efficient and flexible way to enhance immunoassay detection sensitivity and binding kinetics using a nanofluidic based electrokinetic preconcentrator. The device is a microfluidic integration of charge-based biomolecule concentrator and a bead-based immunoassay. Because the preconcentrator can increase the local biomolecule concentration by many orders of magnitude, it gives the immuno-sensor better sensitivity and faster binding kinetics. With a 30 min preconcentration, we were able to enhance the immunoassay sensitivity (with molecular background) by more than 500 fold from higher 50 pM to the sub 100 fM range. Moreover, by adjusting the preconcentration time, we can switch the detection range of the given bead-based assay (from 1010 000 ng ml(-1) to 0.01-10000 ng ml(-1)) to have a broader dynamic range of detection. As the system can enhance both detection sensitivity and dynamic range, it can be used to address the most critical detection issues in the detection of common disease biomarkers.