Tunable, Photoreactive Hydrogel System To Probe Synergies between Mechanical and Biomolecular Cues on Adipose-Derived Mesenchymal Stem Cell Differentiation
Tunable, Photoreactive Hydrogel System To Probe Synergies between Mechanical and Biomolecular Cues on Adipose-Derived Mesenchymal Stem Cell Differentiation
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DOI：
10.1021/acsbiomaterials.5b00196
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发表时间：
2015-08-01
影响因子：
5.8
通讯作者：
Bailey, Ryan C.
中科院分区：
文献类型：
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作者：
Banks, Jessica M.;Harley, Brendan A. C.;Bailey, Ryan C.
将光反应性分子二苯甲酮掺入聚丙烯酰胺水凝胶中，允许对生物分子的空间掺入和基质刚度的选择性调制进行正交控制。将脂肪来源的间充质干细胞在基质上培养，所述基质的弹性模量被调节至与固定化生长因子骨形态发生蛋白2（BMP-2）组合的类似于5、类似于14和类似于37 kPa，并确定它们的谱系分化。观察到BMP-2在中等刚度下具有最显著的效果，而最低和最高刚度水凝胶仅由弹性引导。总之，这种方法描述了一个简单的平台，用于在机械和生化线索的背景下对细胞命运决定进行基础研究，并可能导致干细胞疗法的洞察力和有效性的提高。
The incorporation of the photoreactive molecule benzophenone into polyacrylamide hydrogels, allowing for orthogonal control over spatial incorporation of biomolecules and selective modulation of matrix stiffness, is described. Adipose-derived mesenchymal stem cells were cultured on matrices whose elastic moduli are tuned to similar to 5, similar to 14 and similar to 37 kPa combined with an immobilized growth factor, bone morphogenic protein 2 (BMP-2), and their lineage differentiation was determined. BMP-2 was observed to have the most pronounced effect at the intermediate stiffness, while the lowest and highest stiffness hydrogels are directed by elasticity alone. Together, this approach describes a facile platform for fundamental studies of cell fate decisions in the context of both mechanical and biochemical cues and may lead to improved insight and effectiveness of stem cell therapies.