CATHEPSIN-B, CATHEPSIN-H AND CATHEPSIN-L ACTIVITIES IN GINGIVAL CREVICULAR FLUID FROM CHRONIC ADULT PERIODONTITIS PATIENTS AND EXPERIMENTAL GINGIVITIS SUBJECTS
CATHEPSIN-B, CATHEPSIN-H AND CATHEPSIN-L ACTIVITIES IN GINGIVAL CREVICULAR FLUID FROM CHRONIC ADULT PERIODONTITIS PATIENTS AND EXPERIMENTAL GINGIVITIS SUBJECTS
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DOI：
10.1111/j.1600-0765.1990.tb00894.x
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发表时间：
1990-03-01
影响因子：
3.5
通讯作者：
KATO, I
中科院分区：
文献类型：
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作者：
KUNIMATSU, K;YAMAMOTO, K;KATO, I
为探讨溶酶体半胱氨酸蛋白酶组织蛋白酶B、H和L在牙周组织病理破坏过程中的作用，测定了慢性牙周炎患者和实验性牙龈炎患者龈沟液（GCF）中三种酶的活性。在牙周炎患者中，组织蛋白酶B、H和L活性在疾病活动更严重的部位被发现。每种酶的总活性（单位时间）与GCF体积呈正相关。然而，它与探测深度（PD）几乎没有相关性。相反，GCF中每种酶的比活性（每mg蛋白质的活性单位），反映了酶分泌的选择性，与GCG体积呈负相关。这些结果表明，半胱氨酸蛋白酶的选择性释放到龈沟在相对温和的阶段牙周炎。在实验对象中，没有显着的活性，每种酶的GCF中检测到，即使当GCF的量是从牙周炎患者相媲美。这些数据表明，没有显着量的这些酶被释放在实验牙龈炎网站或稳态机制，包括蛋白酶抑制剂的调节，可能会控制这些酶的活性，在GCG与急性炎症。
To clarify roles of lysosomal cysteine proteinases cathepsin B, H and L in pathological destructive process of periodontal tissues, levels of their enzymatic activities were determined in gingival crevicular fluid (GCF) from chronic adult periodontitis patients and from experimental gingivitis subjects. In periodontitis patients, higher levels of cathepsins B, H and L activities were found at sites with more serious signs of the disease activity. The total activity of each enzyme (per unit time) was positively correlated with the GCF volume. However, it had little or no correlation with the probing depth (PD). In contrast, the specific activity of each enzyme in GCF (activity units per mg protein), which reflects the selectivity of enzyme exudation, was negatively correlation with the GCG volume. These results suggest that the cysteine proteinases are selectively released into gingival crevices at a relatively mild stage of periodontitis. In experimental subjects, no significant activity of each enzyme was detected in GCF, even when the quantity of GCF was comparable to that from periodontitis patients. These data suggest that no significant amounts of these enzymes are released at experimental gingivitis sites or that a homeostatic mechanism, including regulation by protease inhibitors, may control activities of these enzymes in GCG with acute inflammation.