Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly.
Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly.
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DOI：
10.1083/jcb.118.1.109
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发表时间：
1992-07
期刊：
影响因子：
--
通讯作者：
Saunders WS
中科院分区：
文献类型：
--
作者：
Hoyt MA;He L;Loo KK;Saunders WS
两个酿酒酵母基因CIN8和KIP1（又名CIN9）因其需要正常染色体分离而被鉴定出来。这两个基因都编码与微管产力酶激酶重链相关的多肽。在37℃有丝分裂纺锤体组装过程中，Cin8p被发现是极点分离所必需的，尽管过量生产的Kip1p可以替代。在较低的温度下，这些蛋白质中至少有一种的活性是细胞生存所必需的，这表明它们执行必要但冗余的功能。Cin8p被观察到是有丝分裂纺锤体的一个组成部分，与位于两极之间的微管共定位。综上所述，这些发现表明，这些蛋白质与纺锤体微管相互作用，产生一个向外的力作用在极点上。
Two Saccharomyces cerevisiae genes, CIN8 and KIP1 (a.k.a. CIN9), were identified by their requirement for normal chromosome segregation. Both genes encode polypeptides related to the heavy chain of the microtubule- based force-generating enzyme kinesin. Cin8p was found to be required for pole separation during mitotic spindle assembly at 37 degrees C, although overproduced Kip1p could substitute. At lower temperatures, the activity of at least one of these proteins was required for cell viability, indicating that they perform an essential but redundant function. Cin8p was observed to be a component of the mitotic spindle, colocalizing with the microtubules that lie between the poles. Taken together, these findings suggest that these proteins interact with spindle microtubules to produce an outwardly directed force acting upon the poles.