The Effects of Exogenous Ascorbic Acid on the Mechanism of Physiological and Biochemical Responses to Nitrate Uptake in Two Rice Cultivars (Oryza sativa L.) Under Aluminum Stress (vol 35, pg 1013, 2016)
The Effects of Exogenous Ascorbic Acid on the Mechanism of Physiological and Biochemical Responses to Nitrate Uptake in Two Rice Cultivars (Oryza sativa L.) Under Aluminum Stress (vol 35, pg 1013, 2016)
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外源抗坏血酸对铝胁迫下两个水稻品种（Oryza sativa L.）硝酸盐吸收生理生化反应机制的影响（第 35 卷，第 1013 页，2016 年）
DOI：
10.1007/s00344-016-9599-9
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发表时间：
2017
影响因子：
4.8
通讯作者：
Li Kunzhi
中科院分区：
文献类型：
--
作者：
Zhou Xiaohua;Gu Zhaohu;Xu Huini;Chen Limei;Tao Guangxi;Yu Yongxiong;Li Kunzhi
抗坏血酸（阿萨）作为植物体内的主要抗氧化剂，在植物对铝胁迫的响应中起着非常重要的作用。然而，阿萨对铝毒害的缓解作用以及铝胁迫下植物吸收硝态氮（NO3−-N）的机制尚不清楚。以抗铝性较弱的峰1 A（不育系，籼稻）和抗铝性较强的峰1上级5（杂交F1，籼稻）为材料，采用水培试验研究了外源阿萨对水稻根系吸收NO3--N的生理生化反应。铝胁迫导致H2 O2和丙二醛（MDA）含量增加，超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）等抗氧化酶活性升高。铝胁迫下，水稻根系质膜H+-ATPase和H+-泵活性、内源阿萨含量和NO3−-N吸收量均下降。2 mmol L-1外源阿萨与Al复合处理后，水稻根系H2 O2和MDA含量显著降低，根系内源阿萨含量和SOD、POD、CAT、APX活性显著升高;此外，与未加阿萨处理的对照相比，PM H+-ATPase与14-3-3蛋白的相互作用也显著增强，这明显增加了NO3−-N的吸收。结果表明，施用阿萨可显著降低Al胁迫下水稻根系H2 O2和MDA的积累，提高根系质膜H+-ATPase和H+-泵活性，提高抗氧化酶活性，增强质膜H+-ATPase与14-3-3蛋白的相互作用，从而促进水稻对NO3−-N的吸收。
As a major antioxidant in plants, ascorbic acid (AsA) plays a very important role in the response to aluminum (Al) stress. However, the effect of AsA on the mitigation of Al toxicity and the mechanism of nitrate nitrogen (NO3−–N) uptake by plants under Al stress are unclear. In this study, a hydroponic experiment was conducted using peak 1 A rice (sterile line, Indica) with weaker resistance to Al and peak 1 superior 5 rice (F1 hybrid, Indica) with stronger resistance to Al to study the effects of exogenous AsA on the physiological and biochemical responses to NO3−–N uptake by rice roots exposed to 50 μmol L−1Al. Al stress induced increases in the concentrations of H2O2and malondialdehyde (MDA) and in the activities of antioxidant enzymes [such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX)]. Plasma membrane (PM) H+-ATPase and H+-pump activities, endogenous AsA content and NO3−–N uptake in rice roots decreased under Al stress. After treatment with 2 mmol L−1exogenous AsA combined with Al, concentrations of H2O2and MDA in roots notably decreased, and endogenous AsA content and activities of SOD, POD, CAT, and APX in rice roots increased significantly; furthermore, the interaction of PM H+-ATPase and the 14-3-3 protein was also enhanced significantly compared with that in control plants without AsA treatment, which clearly increased NO3−–N uptake. Based on all of these data, the application of AsA significantly reduced the accumulation of H2O2and MDA and increased the activities of PM H+-ATPase and the H+-pump by increasing the endogenous AsA content, the antioxidant enzyme activities, and the interaction of PM H+-ATPase and the 14-3-3 protein in the roots of the two rice cultivars under Al stress, thereby improving the uptake of NO3−–N in rice.