THE VACUOLATING VIRUS, SV40
THE VACUOLATING VIRUS, SV40
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DOI：
10.3181/00379727-105-26128
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发表时间：
1960-01-01
影响因子：
--
通讯作者：
HILLEMAN, MR
中科院分区：
文献类型：
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作者：
SWEET, BH;HILLEMAN, MR
新发现的空泡病毒 S.V.40 似乎是恒河猴肾细胞培养物中常见且基本上普遍存在的污染物，并且可能是食蟹猴肾培养物中的常见污染物，在食蟹猴肾培养物中，它发展到高滴度而没有明显的细胞病变变化。该病毒很容易在绿貂、长尾黑颚猴和帕塔斯肾以及恒河猴睾丸培养物中生长，导致以气球状和强烈空泡化为特征的独特细胞病理学。描述了病毒的一些生物学和生物物理特性。人们对空泡病毒的主要兴趣和重要性源于其在所有 3 种萨宾活脊髓灰质炎病毒疫苗和人类志愿者接种实验中使用的众多呼吸道病毒种子系中的存在。该病毒的证实提出了对人类受试者（尤其是婴儿）施用后的安全性问题，以及测量减毒脊髓灰质炎病毒疫苗株的神经毒力或“遗传”标记以及确定呼吸道病毒对人类的致病性的实验得出的结论可能缺乏有效性的问题。空泡病毒由于其对福尔马林的敏感性而在灭活病毒疫苗中得到有效处理。活病毒疫苗问题的最佳解决方案似乎在于从此类制剂中消除病毒。
The newly discovered vacuolating virus, S.V.40, appears to be a common and essentially ubiquitous contaminant of rhesus monkey kidney cell cultures and a likely common contaminant of cynomolgus kidney cultures in which it develops to high titer without evident cytopathic change. The virus grows readily in grivet, vervet and patas kidney and in rhesus testicle cultures causing a unique cytopathology characterized by ballooning and intense vacuolation. Some biological and biophysical properties of the virus are described. Principal interest in and importance of the vacuolating virus arise from its demonstrated presence in all 3 types of Sabin''s live poliovirus vaccines and in numerous respiratory virus seed lines employed in human volunteer inoculation experiments. Demonstration of the virus has raised the question of safety following administration to human subjects, especially infants, and of possible lack of validity of conclusions reached from experiments to measure neurovirulence or "genetic" markers of attenuated poliovirus vaccine strains and to determine the pathogenicity of respiratory viruses for man. The vacuolating virus is effectively dealt with in killed virus vaccines by virtue of its susceptibility to formalin. The optimal solution to the live virus vaccine problem appears to lie in elimination of the virus from such preparations.