Heterogeneity among Ly-49C natural killer (NK) cells: Characterization of highly related receptors with differing functions and expression patterns
Heterogeneity among Ly-49C natural killer (NK) cells: Characterization of highly related receptors with differing functions and expression patterns
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DOI：
10.1084/jem.184.6.2085
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发表时间：
1996-12-01
影响因子：
15.3
通讯作者：
Takei, F
中科院分区：
文献类型：
--
作者：
Brennan, J;Lemieux, S;Takei, F
Ly-49 C是鼠NK细胞抑制性受体多态性家族的成员。5E 6抗体定义了负责F1小鼠对亲本H-2（d）骨髓的排斥的NK细胞亚群，先前已显示5E 6抗体与Ly-49 C反应。在此，发现5E 6抗体检测B6小鼠中的两种Ly-49 C相关分子。从B6 NK细胞中分离到两个cDNA克隆，一个与先前报道的Ly-49 C（B6）相同，另一个是新的cDNA。后者的推导的氨基酸序列与Ly-49 C（BALB）的仅4个残基不同，而先前报道的Ly-49 C（B6）在22个残基上不同。用两种cDNA转染的COS细胞的流式细胞术分析显示，5E 6抗体结合两种Ly-49分子，而另一种抗Ly-49 C抗体4LO 3311结合新描述的Ly-49 C，但不结合先前报道的Ly-49 C（B6）。双色流式细胞术分析检测到来自B6但不是BALB/c小鼠的NR细胞的5E 6（+）4LO 3311（-）以及5E 6（+）4LO 3311（+）亚群。通过4L 03311抗体检测的B6 NK细胞上的Ly-49 C表达水平显著低于BALB/c NK细胞上的Ly-49 C表达水平。新的Ly-49 C（B6）的结合特异性与Ly-49 C（BALB）的结合特异性不可区分，而与先前报道的Ly-49 C（B6）没有检测到结合。这些结果表明，新描述的Ly-49 C（B6），而不是以前报道的Ly-49 C（B6），是Ly-49 C的可能的B6等位基因形式。以前报道的Ly-49 C（B6）必须由一个单独的基因编码，应重新命名为Ly-49 I。这些结果的影响方面的作用，Ly-49 C在杂交抗性进行了讨论。
Ly-49C is a member of the polymorphic family of murine NK cell inhibitory receptors. The 5E6 antibody that defines a subset of NK cells responsible for the rejection of parental H-2(d) bone marrow by Fl mice has been shown previously to react with Ly-49C. Here, the 5E6 antibody was found to detect two Ly-49C-related molecules in B6 mice. Two cDNA clones were isolated from B6 NK cells, one identical to previously reported Ly-49C(B6) and the other a novel cDNA. The deduced amino acid sequence of the latter differs from that of Ly-49C(BALB) at only 4 residues, whereas the previously reported Ly-49C(B6) differs at 22 residues. Flow cytometric analyses of COS cells transfected with the two cDNAs showed that the 5E6 antibody binds to both Ly-49 molecules, while another anti-Ly-49C antibody, 4LO3311, binds to the newly described Ly-49C but not the previously reported Ly-49C(B6). Two-color now cytometric analysis detected 5E6(+)4LO3311(-) as well as 5E6(+)4LO3311(+) subsets of NR cells from B6, but not BALB/c, mice. The level of Ly-49C expression on B6 NK cells detected by the 4LO3311 antibody was substantially lower than that on BALB/c NK cells. Binding specificity of the novel Ly-49C(B6) was indistinguishable from that of Ly-49C(BALB), whereas no binding was detectable with previously reported Ly-49C(B6). These results demonstrate that the newly described Ly-49C(B6), not the previously reported Ly-49C(B6), is the probable B6 allelic form of Ly-49C. The previously reported Ly-49C(B6) must be encoded by a separate gene and should be re named Ly-49I. The implication of these results with respect to the role of Ly-49C in hybrid resistance is discussed.