Downregulation of ING3 mRNA expression predicts poor prognosis in head and neck cancer
Downregulation of ING3 mRNA expression predicts poor prognosis in head and neck cancer
复制标题
DOI：
10.1111/j.1349-7006.2007.00708.x
复制
发表时间：
2008-03-01
期刊：
影响因子：
5.7
通讯作者：
Nagai, Noriyuki
中科院分区：
文献类型：
--
作者：
Gunduz, Mehmet;Beder, Levent Bekir;Nagai, Noriyuki
尽管已经描述了许多临床和病理预后因素，例如肿瘤分期和淋巴结受累，但迄今为止，尚未确定头颈癌的可靠或临床适用的标志物或肿瘤侵袭性。为了鉴定这样一个分子预后标志物，我们通过定量逆转录聚合酶链式反应分析了 ING3 的 mRNA 表达状态。我们还检查了 p53 突变状态并研究了其与 ING3 的关系及其临床病理特征。与匹配的正常样本相比，71 个肿瘤样本中约有一半显示 ING3 下调。尽管大多数临床病理学变量与 ING3 下调或 p53 突变状态没有显着相关，但在 ING3 低表达和正常至高表达病例之间的总生存率方面检测到显着关系。随访 5 年时，ING3 表达正常至高的患者中约有 60% 存活，而 ING3 表达低的患者中这一比例为 35%。多变量分析还显示 ING3 的下调是总体生存率较差的独立预后因素。这些结果表明 ING3 将充当潜在的肿瘤抑制分子，并且低水平的 ING3 可能表明头颈癌的侵袭性。
Although many clinical and pathological prognostic factors such as tumor stage and lymph-node involvement have been described, to date no reliable or clinically applicable marker or tumor aggressiveness has been identified for head and neck cancer. In an attempt to identify such a molecular prognostic marker, we analyzed the mRNA expression status of ING3 by quantitative reverse transcription-polymerase chain reaction. We also examined p53 mutation status and investigated its relationship with ING3, as well its clinicopathological characteristics. About half of the 71 tumor samples demonstrated downregulation of ING3 compared to their matched normal counterparts. Although most clinicopathological variables were not significantly related to ING3 downregulation or p53 mutation status, a significant relationship was detected in terms of overall survival between the cases with low and normal to high ING3 expression. At 5 years follow up, approximately 60% of the patients with normal to high ING3 expression survived, whereas this was 35% in the patients with low ING3 expression. Multivariate analysis also showed downregulation of ING3 as an independent prognostic factor for poor overall survival. These results reveal that ING3 would function as a potential tumor suppressor molecule and that low levels of ING3 may indicate an aggressive nature of head and neck cancer.