A novel DNA-based microfluorimetric method to evaluate antimalarial drug activity
A novel DNA-based microfluorimetric method to evaluate antimalarial drug activity
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DOI：
10.4269/ajtmh.2004.70.119
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发表时间：
2004-02-01
影响因子：
3.3
通讯作者：
Ortega-Barria, E
中科院分区：
文献类型：
--
作者：
Corbett, Y;Herrera, L;Ortega-Barria, E
本文描述了一种基于用PicoGreen（R）嵌入检测寄生虫DNA来测量恶性疟原虫抑制作用的新型微量荧光测定法的开发。该方法用于确定已知或潜在抗疟药物的寄生虫抑制曲线和50%抑制浓度值。使用PicoGreen（R）测定法得到的已知抗疟药物对寄生虫的抑制值与基于H - 3 - 次黄嘌呤摄取和吉姆萨染色显微镜技术的标准方法所测定的值相当。PicoGreen（R）测定法快速、灵敏、可重复、易于解读，非常适合用于抗疟药物研发中大量样本的筛选。
This paper describes the development of a novel microfluorimetric assay to measure the inhibition of Plasmodium falciparum based on the detection of parasitic DNA by intercalation with PicoGreen(R). The method was used to determine parasite inhibition profiles and 50% inhibitory concentration values of known or potential antimalarial drugs. Values for parasite inhibition with known anti-malarial drugs using the PicoGreen(R) assay were comparable with those determined by the standard method based upon the uptake of H-3-hypoxanthine and the Giemsa stain microscopic technique. The PicoGreen(R) assay is rapid, sensitive, reproducible, easily interpreted, and ideally suited for screening of large numbers of samples for anti-malarial drug development.