Urease encapsulation in nanoorganized microshells
Urease encapsulation in nanoorganized microshells
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DOI：
10.1021/nl0100015
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发表时间：
2001-03-01
期刊：
影响因子：
10.8
通讯作者：
Sukhorukov, GB
中科院分区：
文献类型：
--
作者：
Lvov, Y;Antipov, AA;Sukhorukov, GB
通过在三聚氰胺甲醛微核上逐层组装聚烯丙胺（PAH）和聚苯乙烯磺酸（PSS），然后在低ph下进行核分解，制备了稳定的中空聚电解质胶囊。这些胶囊在水中对脲酶不渗透，在水/乙醇混合物中具有渗透性。在水/乙醇混合物中装载脲酶，然后在水中重悬。脲酶分子被保存在胶囊中，而小尿素分子迅速扩散通过胶囊壁，为生物催化反应提供底物。
Stable hollow polyelectrolyte capsules were produced by means of the layer-by-layer assembling of poly(allylamine), PAH, and poly(styrenesulfonate), PSS, on melamine formaldehyde microcores followed by the core decomposition at low pH. These capsules are nonpermeable for urease in water and become permeable in a water/ethanol mixture. The capsules were loaded with urease in water/ethanol mixture and then resuspended in water. The urease molecules are kept in the capsule, whereas the small urea molecules rapidly diffuse through the capsule wall providing a substrate for the biocatalytic reaction.