Replication competent virus as an important source of bias in HIV latency models utilizing single round viral constructs.
Replication competent virus as an important source of bias in HIV latency models utilizing single round viral constructs.
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DOI：
10.1186/s12977-014-0070-3
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发表时间：
2014-08-21
期刊：
影响因子：
3.3
通讯作者：
Vandekerckhove L
中科院分区：
文献类型：
--
作者：
Bonczkowski P;De Spiegelaere W;Bosque A;White CH;Van Nuffel A;Malatinkova E;Kiselinova M;Trypsteen W;Witkowski W;Vermeire J;Verhasselt B;Martins L;Woelk CH;Planelles V;Vandekerckhove L
中央记忆T细胞（TCM）模型基于与体内TCM非常相似的原代细胞形成独特的HIV-1潜伏期模型。该模型中使用的病毒基于在初始感染后不能复制的工程化载体。我们表明，尽管这种策略，复制能力的病毒颗粒被释放到培养基中，由于与完整的env共转染的env缺失的HIV基因组的重叠序列之间的重组。这一发现强调，如果采用类似的结构，需要仔细的数据分析和解释，并敦促在实验室工作中额外的谨慎。本文的在线版本（doi：10.1186/s12977-014-0070-3）包含补充材料，可供授权用户使用。
The central memory T cell (TCM) model forms a unique HIV-1 latency model based on primary cells that closely resemble in vivo TCM. The virus employed in this model is based on an engineered vector incapable of replication after initial infection. We show that despite this strategy, replication competent viral particles are released into the culture medium due to recombination between overlapping sequences of the env deleted HIV genome that is co-transfected with intact env. This finding emphasizes the need for careful data analysis and interpretation if similar constructs are employed and urges for additional caution during laboratory work. The online version of this article (doi:10.1186/s12977-014-0070-3) contains supplementary material, which is available to authorized users.
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影响因子：
20.3
作者：
Bosque, Alberto;Planelles, Vicente
通讯作者：
Planelles, Vicente
DOI：
10.1073/pnas.93.21.11382
发表时间：
1996-10-15
影响因子：
11.1
作者：
Naldini, L;Blomer, U;Verma, IM
通讯作者：
Verma, IM
影响因子：
30.3
作者：
Lusic, Marina;Marini, Bruna;Giacca, Mauro
通讯作者：
Giacca, Mauro
影响因子：
3.3
作者：
Sherrill-Mix S;Lewinski MK;Famiglietti M;Bosque A;Malani N;Ocwieja KE;Berry CC;Looney D;Shan L;Agosto LM;Pace MJ;Siliciano RF;O'Doherty U;Guatelli J;Planelles V;Bushman FD
通讯作者：
Bushman FD
影响因子：
4.8
作者：
Bosque, Alberto;Planelles, Vicente
通讯作者：
Planelles, Vicente