Detection of HER-2 oncogene amplification in breast cancer by differential polymerase chain reaction from single cryosections
Detection of HER-2 oncogene amplification in breast cancer by differential polymerase chain reaction from single cryosections
复制标题
通过单次冰冻切片的差异聚合酶链反应检测乳腺癌中 HER-2 癌基因的扩增
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
H. Höfler
中科院分区：
文献类型：
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作者：
K. Friedrichs;D. Lohmann;H. Höfler
编码癌基因如HER-2（syn. c-erbB 2/c-neu）可能实质上参与乳腺癌的发生和发展。为了完善和促进乳腺癌HER-2扩增的定量分析，对来自肿瘤组织单个冷冻切片的DNA进行差异聚合酶链反应（PCR）。该技术基于潜在扩增的癌基因（HER-2）和参考基因（IFN-γ）的同时扩增。差异PCR产生了可重复的结果，与使用斑点印迹技术的基因拷贝定量一致。因此，我们建议差异PCR是一种可靠和快速的方法来确定相对基因剂量在一个微小的肿瘤组织。
SummaryAmplification of genomic DNA encoding oncogenes such as HER-2 (syn. c-erbB2/c-neu) may be substantially involved in the initiation and progression of breast cancer. In order to refine and facilitate the quantitative analysis of HER-2 amplification in breast cancer, differential polymerase chain reaction (PCR) was performed on DNA derived from single cryosections of tumor tissue. This technique is based on the simultaneous amplification of a potentially amplified oncogene (HER-2) and a reference gene (IFN-gamma). Differential PCR yielded reproducible results that were in agreement with gene copy quantification using the dot blot technique. Thus we suggest differential PCR to be a reliable and rapid method for determining relative gene dosage in a minute amount of tumour tissue.
影响因子：
3.3
作者：
ALLRED, DC;CLARK, GM;MCGUIRE, WL
通讯作者：
MCGUIRE, WL
影响因子：
8
作者：
E. T. Liu;A. Thor;M. He;M. Barcos;B. Ljung;C. Benz
通讯作者：
E. T. Liu;A. Thor;M. He;M. Barcos;B. Ljung;C. Benz