Structures and biological activities of novel phosphatidylethanolamine lipids of Porphyromonas gingivalis
Structures and biological activities of novel phosphatidylethanolamine lipids of Porphyromonas gingivalis
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DOI：
10.1194/jlr.m500542-jlr200
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发表时间：
2006-04-01
影响因子：
6.5
通讯作者：
Smith, MB
中科院分区：
文献类型：
--
作者：
Nichols, FC;Riep, B;Smith, MB
革兰氏阴性牙周病原体牙龈卟啉单胞菌合成几类新型磷酸化复合脂质，包括最近表征的磷酸化二氢神经酰胺。这些鞘脂促进白细胞介素 1 (IL-1) 介导的成纤维细胞分泌炎症介质，包括前列腺素 E-2 和 6-酮前列腺素 F-2 α，并改变培养物中的牙龈成纤维细胞形态。该报告表明，牙龈卟啉单胞菌的另一类磷酸化复合脂质可促进培养的成纤维细胞中 IL-1 介导的分泌反应和形态变化。结构表征确定新的磷脂类别为 1,2-二酰基磷脂酰乙醇胺，它主要被异支链 C-15:0 和 C-13:0 脂肪酸取代。异支链脂肪酸（而不是直链脂肪酸）和磷酸乙醇胺头基被确定为促进 IL-1 介导的分泌反应所需的基本结构元件。除了本报告中描述的磷脂酰乙醇胺脂质之外，这些结构成分也在牙龈卟啉单胞菌的特定磷酸化鞘脂中观察到，并且可能有助于这些物质的生物活性。
The Gram-negative periodontal pathogen Porphyromonas gingivalis synthesizes several classes of novel phosphorylated complex lipids, including the recently characterized phosphorylated dihydroceramides. These sphingolipids promote the interleukin-1 ( IL-1)-mediated secretion of inflammatory mediators from fibroblasts, including prostaglandin E-2 and 6-keto prostaglandin F-2 alpha, and alter gingival fibroblast morphology in culture. This report demonstrates that one additional class of phosphorylated complex lipids of P. gingivalis promotes IL-1-mediated secretory responses and morphological changes in cultured fibroblasts. Structural characterization identified the new phospholipid class as 1,2-diacyl phosphatidylethanolamine, which substituted predominantly with isobranched C-15:0 and C-13:0 fatty acids. The isobranched fatty acids, rather than unbranched fatty acids, and the phosphoethanolamine head group were identified as the essential structural elements required for the promotion of IL-1-mediated secretory responses. These structural components are also observed in specific phosphorylated sphingolipids of P. gingivalis and likely contribute to the biological activity of these substances, in addition to the phosphatidylethanolamine lipids described in this report.