CHARACTERIZATION OF THE MEMBRANE-PROTEINS OF RAT-LIVER LYSOSOMES - COMPOSITION, ENZYME-ACTIVITIES AND TURNOVER
CHARACTERIZATION OF THE MEMBRANE-PROTEINS OF RAT-LIVER LYSOSOMES - COMPOSITION, ENZYME-ACTIVITIES AND TURNOVER
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DOI：
10.1042/bj2040525
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发表时间：
1982-01-01
影响因子：
4.1
通讯作者：
SCHNEIDER, DL
中科院分区：
文献类型：
--
作者：
BURNSIDE, J;SCHNEIDER, DL
从未经处理的大鼠肝脏和注射Triton WR-1339或葡聚糖的大鼠肝脏制备的溶酶体产生的膜在多肽组成和ATPase和酸性5‘-核苷酸酶活性方面都是相似的。给予Triton WR-1339(和葡聚糖)可引起肝组织匀浆ATPase活性增加，这与溶酶体膜ATPase活性平行增加有关。在多肽组成和酶活性方面，质膜似乎不同于溶酶体膜。质膜ATPase的抑制剂哇巴因和苏拉明不影响溶酶体膜的ATPase活性。质膜碱性5‘’-核苷酸酶在酸性条件下活性不高。溶酶体膜用[~3H]岩藻糖和[~3H]-和[14C]-亮氨酸脉冲标记的速度比用质膜标记的速度快。标记动力学表明，溶酶体膜可能是独立于质膜组装的。显然，在肝脏中，质膜向溶酶体的大量转运很少发生，溶酶体膜蛋白可能不是来自质膜的蛋白。
Lysosomes prepared from the livers of untreated rats and from the livers of rats injected with either Triton WR-1339 or dextran yielded membranes that were similar in both polypeptide composition and activities of ATPase and acid 5''-nucleotidase. The administration of Triton WR-1339 (and dextran) resulted in an increase in ATPase activity of liver homogenates that was associated with a parallel increase in the ATPase activity of the lysosomal membrane. Plasma membranes appear to be different from lysosomal membranes with respect to polypeptide composition and enzyme activities. The ATPase activity of lysosomal membranes is not affectd by ouabain and suramin, inhibitors of the plasma-membrane ATPase. The plasma-membrane alkaline 5''-nucleotidase has little activity at acid pH. Pulse-labeling of lysosomal membranes with [3H]fucose and with [3H]- and [14C]-leucine occurred rapidly, faster than labeling of plasma membranes. The labeling kinetics indicate that lysosomal membranes may be assembled independently of plasma membranes. Apparently, in liver, little bulk transport of plasma membrane to lysosomes takes place, and lysosomal-membrane proteins may not be derived from those of plasma membranes.