Three-dimensional Ca2+ imaging advances understanding of astrocyte biology
Three-dimensional Ca2+ imaging advances understanding of astrocyte biology
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DOI：
10.1126/science.aai8185
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发表时间：
2017-05-19
期刊：
影响因子：
56.9
通讯作者：
Volterra, Andrea
中科院分区：
文献类型：
--
作者：
Bindocci, Erika;Savtchouk, Iaroslav;Volterra, Andrea
星形胶质细胞通信通常通过二维钙离子（Ca 2+）成像进行研究，但这种方法尚未获得星形胶质细胞在突触和血管功能中的作用的结论性数据。我们开发了一种三维双光子成像方法，并研究了整个星形胶质细胞体积中的Ca 2+动力学，包括轴突-星形胶质细胞相互作用期间。在清醒的小鼠和脑切片中，我们发现单个星形胶质细胞中的Ca 2+活性分散在整个细胞中，主要分布在区域之间，主要分布在区域内，并且在区域和局部分布不均。过程和终足显示频繁的快速活动，而索马很少活跃。在清醒的小鼠中，活动高于脑切片，特别是在尾足和过程中，并显示偶尔的多灶性细胞范围内的事件。星形胶质细胞局部响应最小轴突发射与时间相关的Ca 2+斑点。
Astrocyte communication is typically studied by two-dimensional calcium ion (Ca2+) imaging, but this method has not yielded conclusive data on the role of astrocytes in synaptic and vascular function. We developed a three-dimensional two-photon imaging approach and studied Ca2+ dynamics in entire astrocyte volumes, including during axon-astrocyte interactions. In both awake mice and brain slices, we found that Ca2+ activity in an individual astrocyte is scattered throughout the cell, largely compartmented between regions, preponderantly local within regions, and heterogeneously distributed regionally and locally. Processes and endfeet displayed frequent fast activity, whereas the soma was infrequently active. In awake mice, activity was higher than in brain slices, particularly in endfeet and processes, and displayed occasional multifocal cellwide events. Astrocytes responded locally to minimal axonal firing with time-correlated Ca2+ spots.