Induction of lymphomas by inoculation of Marek's disease virus-derived lymphoblastoid cell lines: prevention by CVI988 vaccination.
Induction of lymphomas by inoculation of Marek's disease virus-derived lymphoblastoid cell lines: prevention by CVI988 vaccination.
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通过接种马立克氏病病毒衍生的淋巴母细胞系诱导淋巴瘤：通过 CVI988 疫苗接种进行预防。
DOI：
10.1080/03079457.2012.740159
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Mwangi WN
中科院分区：
文献类型：
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作者：
Mwangi WN
淋巴母细胞样细胞系265（L）和990（O）是分别从马立克氏病病毒（MDV）RB-1B株和pRB-1B 5 BAC克隆感染的近交系P系（MHC B19/B19）鸡的肝脏和卵巢肿瘤中产生的单克隆淋巴瘤。通过静脉内或腹腔内途径将其接种到近交、MDV易感、P系鸡中。在接种细胞系之前8天，使用1000噬斑形成单位的CVI 988疫苗接种另外的鸟组。未接种疫苗的鸟类在接种后30至60天出现内脏马立克氏病肿瘤的发病率增加。疫苗接种预防了受攻击鸟类的肿瘤和疾病发展。通过接种物的光谱分析和测序进行的TCRβ库分析用于追踪来自接种禽的原发性肿瘤和肿瘤细胞系中的肿瘤身份。这些数据表明，肿瘤是新生病毒感染的结果，而不是接种的肿瘤细胞的转移和扩增。此外，数据显示，这两种MDV衍生的细胞系即使在同系P系鸟类中也不可移植。这些数据还证明了频谱分析作为一种工具，以跟踪肿瘤的身份在淋巴瘤移植研究中的应用。
Lymphoblastoid cell lines 265(L) and 990(O) are monoclonal lymphomas, derived respectively from liver and ovarian tumours, generated in inbred P-line (MHC B19/B19) chickens infected with RB-1B strain of Marek's disease virus (MDV) and pRB-1B5 BAC clone respectively. These were inoculated into inbred, MDV-susceptible, P-line chickens by intra-venous or intra-abdominal routes. Additional groups of birds were vaccinated using 1000 plaque-forming units of CVI988 vaccine 8 days prior to inoculation of the cell lines. Non-vaccinated birds developed visceral Marek's disease tumours with an increased rate 30 to 60 days post inoculation. Vaccination prevented tumour and disease development in challenged birds. TCRβ repertoire analysis by spectratyping and sequencing of the inoculum was used to track tumour identity in primary tumours and tumour cell lines derived from inoculated birds. These data revealed that the tumours were a consequence ofde novovirus infection and not metastasis and expansion of the inoculated tumour cells. Moreover, the data showed that the two MDV-derived cell lines were not transplantable even in syngeneic P-line birds. The data also demonstrated the application of spectratyping as a tool to track tumour identity in lymphoma transplantation studies.