Antibiofouling amphiphilic polymer-coated superparamagnetic iron oxide nanoparticles: synthesis, characterization, and use in cancer imaging in vivo
Antibiofouling amphiphilic polymer-coated superparamagnetic iron oxide nanoparticles: synthesis, characterization, and use in cancer imaging in vivo
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DOI：
10.1039/b902445j
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发表时间：
2009-01-01
影响因子：
--
通讯作者：
Jon, Sangyong
中科院分区：
文献类型：
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作者：
Park, Jinho;Yu, Mi Kyung;Jon, Sangyong
超顺磁性氧化铁纳米粒子（SPIONs）被广泛用作磁共振成像（MRI）的T2造影剂。在此，我们使用一步纳米乳液法开发了多种抗生物污染的两亲性聚合物包覆的SPIONs。这种方法制备出了平均直径小于30纳米的超小聚合物包覆的SPIONs，它们在生理条件下是稳定的。体外细胞毒性试验表明，即使在相对较高的浓度下，SPIONs也没有显示出毒性。对患有Lewis肺癌（LLC）的荷瘤小鼠进行体内MRI检查，结果显示肿瘤组织中的T2信号下降了约30%，这表明SPIONs通过被动靶向到达了这些组织。总之，超小、稳定的两亲性聚合物包覆的SPIONs可用作癌症成像的MRI造影剂。
Superparamagnetic iron oxide nanoparticles (SPIONs) are widely used as T2-contrast agents for magnetic resonance imaging (MRI). Herein we develop various antibiofouling amphiphilic polymer-coated SPIONs using a one-step nanoemulsion method. This methodology yielded ultrasmall polymer-coated SPIONs, of average diameter less than 30 nm, which were stable under physiological conditions. In vitro cell cytotoxicity tests revealed that no SPION showed toxicity even at relatively high concentrations. In vivo MRI with Lewis lung carcinoma (LLC) tumor-bearing mice resulted in an approximately 30% T2 signal drop in tumor tissues, indicating that the SPIONs reached such tissues via passive targeting. In summary, the ultrasmall, stable, amphiphilic polymer-coated SPIONs can be used as MRI contrast agents for cancer imaging.