Useable diffraction data from a multiple microdomain-containing crystal of Ascaris suum As-p18 fatty-acid-binding protein using a microfocus beamline.
Useable diffraction data from a multiple microdomain-containing crystal of Ascaris suum As-p18 fatty-acid-binding protein using a microfocus beamline.
复制标题
使用微焦点光束线从含有多个微结构域的猪蛔虫 As-p18 脂肪酸结合蛋白晶体中获得可用的衍射数据。
DOI：
10.1107/s1744309112026553
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Gabrielsen M
中科院分区：
文献类型：
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作者：
Gabrielsen M
As-p18 是一种来自寄生线虫蛔虫的脂肪酸结合蛋白。尽管它与哺乳动物细胞内脂肪酸结合蛋白具有序列相似性，但它包含线虫独有的特征。获得了晶体，但最初的衍射数据分析表明它们由许多“微域”组成。只能使用光束尺寸为 12 × 8 μm 的微焦点光束线来收集可解释的数据。
As-p18 is a fatty-acid-binding protein from the parasitic nematode Ascaris suum. Although it exhibits sequence similarity to mammalian intracellular fatty-acid-binding proteins, it contains features that are unique to nematodes. Crystals were obtained, but initial diffraction data analysis revealed that they were composed of a number of `microdomains'. Interpretable data could only be collected using a microfocus beamline with a beam size of 12 × 8 µm.