The Autographa californica M nucleopolyhedrovirus fibroblast growth factor accelerates host mortality
The Autographa californica M nucleopolyhedrovirus fibroblast growth factor accelerates host mortality
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DOI：
10.1016/j.virol.2007.03.027
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发表时间：
2007-08-15
期刊：
影响因子：
3.7
通讯作者：
Passarelli, A. Lorena
中科院分区：
文献类型：
--
作者：
Detvisitsakun, Chanitchote;Cain, Erica L.;Passarelli, A. Lorena
由加利福尼亚Autographa california M核多角体病毒（AcMNPV）编码的成纤维细胞生长因子（vfgf）基因已被证明与细胞fgf具有相同的功能特性；它是一种分泌蛋白，结合肝素，并刺激昆虫细胞的运动。我们以前报道过，含有或缺乏vfgf的病毒在培养细胞中产生相似的出芽病毒产量，并且具有相似的病毒DNA和蛋白质合成动力学。在这项研究中，我们利用昆虫的两个发育阶段和两种感染途径，通过摄食和腹腔内注射，鉴定了这些病毒在两个允许宿主——狐尾翅目和毛虫身上的特征。此外，我们构建了一个在多面蛋白启动子控制下过表达vfgf的AcMNPV bacmid，并在细胞培养和昆虫中对其进行了表征。缺失vfgf对AcMNPV的传染性没有影响，然而，在两种宿主中，当病毒通过喂食传递而不是通过腹腔内注射传递时，缺乏vfgf会延迟死亡时间。过表达vfgf的病毒在培养细胞中产生的出芽病毒比对照病毒少。在昆虫生物测定中，该病毒在两种昆虫中的传染性都大于亲本病毒，并显著加快了两种被测宿主的死亡时间。我们的研究结果表明，AcMNPV vfgf可能在被试昆虫中肠病毒感染的传播中起作用。(c) 2007爱思唯尔公司版权所有。
The fibroblast growth factor (vfgf) gene encoded by Autographa californica M nucleopolyhedrovirus (AcMNPV) has been shown to share functional properties with cellular fgfs; it is a secreted protein, binds heparin, and stimulates motility of insect cells. We previously reported that viruses containing or lacking vfgf produced similar yields of budded virus and had similar kinetics of viral DNA and protein syntheses in cultured cells. In this study, we characterized these viruses in two permissive hosts, Spodoptera frugiperda and Trichoplusia ni, using two insect developmental stages and two infection routes, by feeding and intrahemocoelic injection. In addition, we constructed an AcMNPV bacmid overexpressing vfgf under polyhedrin promoter control and characterized it in both cell culture and insects. Deletion of vfgf had no effect on the infectivity of AcMNPV However, lack of vfgf delayed the time of death in two host species when the virus was delivered by feeding but not by intrahemocoelic injection. The virus overexpressing vfgf produced less budded virus than the control virus in cultured cells. In insect bioassays, the infectivity of this virus was greater than that of the parental virus in both insect species and significantly accelerated time of death of both hosts tested. Our results suggest that the AcMNPV vfgf may play a role in dissemination of virus infection from the midgut in the insect species tested. (c) 2007 Elsevier Inc. All rights reserved.