Effects of rhizobacteria on the respiration and growth of Cerasus sachalinensis Kom. seedlings
Effects of rhizobacteria on the respiration and growth of Cerasus sachalinensis Kom. seedlings
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DOI：
10.5424/sjar/2016142-6848
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发表时间：
2016-06
影响因子：
0.9
通讯作者：
S. Qin;Wenjie Zhou;Zhixia Li;D. Lyu
中科院分区：
文献类型：
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作者：
S. Qin;Wenjie Zhou;Zhixia Li;D. Lyu
在本研究中，我们研究了根际微生物对樱桃种子萌发和根代谢的影响。我们用从根际分离的优势细菌菌株的悬浮液接种了 C. sachalinensis 植物。从高效根部定植微生物中筛选出4株具有显着促生长或生长抑制作用的菌株。根际细菌处理后幼苗根际放线菌数量显着增加，真菌数量显着减少。促生长细菌轻微影响呼吸速率和呼吸途径酶，但显着提高根系活力、根系碳水化合物浓度和幼苗生长。蜡样芽孢杆菌、葡萄球菌属。荧光假单胞菌被鉴定为促生长根际细菌；无法鉴定出一种菌株。接种生长抑制菌后，幼苗根际真菌菌落数量增加，根系活力和呼吸速率以及根部淀粉和蔗糖积累显着降低。接种生长抑制菌后，糖酵解、磷酸戊糖和替代氧化酶途径成为呼吸代谢的主要途径。在接种生长抑制细菌的植物中，幼苗的高度、叶数、生长和干重显着下降。接种促生菌和抑制菌对大竹蝽根际微生物群组成、养分浓度和幼苗生物量等土壤环境因子产生影响。
In this study, we investigated the influence of rhizosphere microorganisms on seed germination and root metabolism in Cerasus sachalinensis Kom. We inoculated C. sachalinensis plants with suspensions of dominant bacterial strains isolated from their rhizosphere. Four bacterial strains each with significant growth-promoting or growth-inhibiting effects were screened from the efficient root-colonizing microorganisms. The number of actinomycetes increased and that of fungi decreased significantly in the seedling rhizospheres after rhizobacteria treatment. The growth-promoting bacteria slightly affected the respiration rates and respiratory pathway enzymes, but significantly improved root viability, root carbohydrate concentration and seedling growth. Bacillus cereus , Staphylococcus sp. and Pseudomonas fluorescens were identified as the growth-promoting rhizobacteria; one strain could not be identified. After inoculation with the growth-inhibiting bacteria, the number of fungal colonies in the seedling rhizospheres increased and root viability and respiration rate as well as starch and sucrose accumulation in the roots significantly decreased. The glycolysis, pentose phosphate and alternative oxidase pathways became the major pathways of respiratory metabolism after inoculation with the growth-inhibiting bacteria. The height, leaf number, growth and dry weight of the seedlings decreased significantly in plants inoculated with the growth-inhibiting bacteria. Inoculation of C. sachalinensis rhizosphere with growth-promoting and growth-inhibiting bacteria affected the soil environmental factors such as microbial group composition, nutrient concentration and seedling biomass.