Optical coherence tomography for optical biopsy - Properties and demonstration of vascular pathology
Optical coherence tomography for optical biopsy - Properties and demonstration of vascular pathology
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DOI：
10.1161/01.cir.93.6.1206
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发表时间：
1996-03-15
期刊：
影响因子：
37.8
通讯作者：
Fujimoto, JG
中科院分区：
文献类型：
--
作者：
Brezinski, ME;Tearney, GJ;Fujimoto, JG
背景光学相干层析成像(OCT)是近年来发展起来的一种医学诊断技术，它利用背向反射红外光进行微米级的原位层析成像。在本工作中，我们研究了OCT对体外动脉粥样硬化斑块内部微结构进行微米级断层成像的能力。方法和结果尸检时获取主动脉及相关非血管组织。用OCT进行动脉节段外露表面的二维断层成像。采用波长为1300 nm的超发光二极管光源，其轴向空间分辨率为20微米，信噪比为109分贝。图像以灰度级或假彩色显示，对严重钙化的组织进行超过1.5 mm的成像，并注意到脂类和水基成分之间的高对比度，使OCT对冠状动脉成像具有吸引力。OCT的轴向分辨率为20微米，可以确定微小的结构细节，如内膜帽的宽度和裂隙的存在。后向散射强度低的脂质聚集程度也得到了很好的记录。结论OCT是一种很有前途的血管微结构成像新技术，其分辨率是其他成像方式所无法达到的。它不需要与血管壁直接接触，并且可以通过与相对便宜的光纤集成的导管来进行。组织成分之间的高对比度、高分辨率和穿透严重钙化组织的能力使OCT成为冠状动脉内诊断的一种有吸引力的新成像技术。
Background Optical coherence tomography (OCT) is a recently developed medical diagnostic technology that uses back-reflected infrared light to perform in situ micron scale tomographic imaging. In this work, we investigate the ability of OCT to perform micron scale tomographic imaging of the internal microstructure of in vitro atherosclerotic plaques.Methods and Results Aorta and relevant nonvascular tissue were obtained at autopsy. Two-dimensional cross-sectional imaging of the exposed surface of the arterial segments was performed in vitro with OCT. A 1300-nm wavelength, super-luminescent diode light source was used that allows an axial spatial resolution of 20 mu m. The signal-to-noise ratio was 109 dB. Images were displayed in gray scale or false color, Imaging was performed over 1.5 mm into heavily calcified tissue, and a high contrast was noted between lipid- and water-based constituents, making OCT attractive for intracoronary imaging. The 20-mu m axial resolution of OCT allowed small structural details such as the width of intimal caps and the presence of fissures to be determined. The extent of lipid collections, which had a low backscattering intensity, also were well documented.Conclusions OCT represents a promising new technology for imaging Vascular microstructure with a level of resolution not previously achieved with the use of other imaging modalities. It does not require direct contact with the vessel wall and can be performed with a catheter integrated with a relatively inexpensive optical fiber. The high contrast among tissue constituents, high resolution, and ability to penetrate heavily calcified tissue make OCT an attractive new imaging technology for intracoronary diagnostics.