MicroRNA-126 affects ovarian cancer cell differentiation and invasion by modulating expression of vascular endothelial growth factor
MicroRNA-126 affects ovarian cancer cell differentiation and invasion by modulating expression of vascular endothelial growth factor
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MicroRNA-126通过调节血管内皮生长因子的表达影响卵巢癌细胞的分化和侵袭
DOI：
10.3892/ol.2018.8025
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发表时间：
2018-04-01
期刊：
影响因子：
2.9
通讯作者：
Zhou, Jianwei
中科院分区：
文献类型：
--
作者：
Luo, Jianqiao;Zhu, Caidan;Zhou, Jianwei
原发性卵巢癌是妇科癌症相关死亡的主要原因。然而，卵巢癌扩散背后的机制需要阐明。本研究旨在探讨microRNA-126（miR-126）对卵巢癌分化和侵袭的影响及其机制。制备了转染LV 3-has-miR-126 mimics和LV 3-has-miR-126抑制剂的卵巢癌SKOV 3细胞，发现LV-miR-126 mimics可诱导细胞周期停滞在G（1）期，抑制细胞通过Matrigel包被膜的侵袭，并下调血管内皮生长因子（VEGF）的表达。此外，转染LV-has-miR-126的细胞可增加S期细胞数，诱导细胞侵袭，并上调VEGF的表达。据我们所知，本研究首次报道了miR-126可能通过诱导G（1）细胞周期阻滞和抑制卵巢癌细胞的侵袭，至少部分通过靶向VEGF表达来发挥肿瘤抑制作用。
Primary ovarian cancer is the main cause of gynecological cancer-associated mortality. However, the mechanism behind the spread of ovarian cancer requires elucidation. The present study aimed to investigate the effects of microRNA-126 (miR-126) on differentiation and invasion, and its mechanism in primary ovarian cancer. Ovarian cancer SKOV3 cells transfected with LV3-has-miR-126 mimics and LV3-has-miR-126 inhibitor were produced; it was revealedthatLV-miR-126 mimics could induce cell cycle arrest at G(1) phase, suppress cell invasion through Matrigel-coated membranes and downregulate the expression of vascular endothelial growth factor (VEGF). Furthermore, LV-has-miR-126 inhibitor-transfected cells could increase the number of cells in S phase, induce cell invasion and upregulate the expression of VEGF. The present study, to the best of our knowledge, is the first to report that miR-126 may serve tumor suppressor roles by inducing G(1) cell cycle arrest and suppressing invasion in ovarian cancer cells, at least in part by targeting VEGF expression.