THE OCCURRENCE OF INTERGLOBULAR DENTIN IN INCISORS OF HYPOPHOSPHATEMIC MICE FED A HIGH-CALCIUM AND HIGH-PHOSPHATE DIET
THE OCCURRENCE OF INTERGLOBULAR DENTIN IN INCISORS OF HYPOPHOSPHATEMIC MICE FED A HIGH-CALCIUM AND HIGH-PHOSPHATE DIET
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DOI：
10.1177/00220345920710031101
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发表时间：
1992-03-01
影响因子：
7.6
通讯作者：
OOSHIMA, T
中科院分区：
文献类型：
--
作者：
ABE, K;MASATOMI, Y;OOSHIMA, T
对低磷血症(Hyp)小鼠切牙牙本质进行了组织病理学检查，以确定血清磷水平是否会影响小球间牙本质的多发性。将正常小鼠和12周龄Hyp小鼠随机分为两组。其中一组给予对照组饲料(含钙1.42%，含磷1.16%)，另一组给予高钙高磷饲料(含钙2.00%，含磷3.00%)，共30天。每隔第五天从小鼠身上采集血液，测量血清中钙和磷的浓度。磨碎切片和脱钙切片均取自下颌和上颌的门牙，用于显微镜检查。正常小鼠的血清钙和磷水平几乎不变，与饮食无关。另一方面，饲喂对照饲料的Hyp小鼠的血清P水平显著低于正常小鼠。饲喂高钙磷饲料10天后，Hyp小鼠的血清P水平显著升高，接近正常小鼠水平。组织病理学检查显示，高钙磷饲料喂养的Hyp小鼠切牙牙本质无明显变化，仍有球状牙本质形成。这些结果表明，作为X连锁低磷血症性维生素D抗性软骨病最显著特征的球间牙本质，在Hyp小鼠体内不受血磷水平短期归一化的影响。
The incisor dentin of hypophosphatemic (Hyp) mice was examined histopathologically to determine whether the multiple occurrences of interglobular dentin would be influenced by the serum phosphate level. Both normal and Hyp mice (12 weeks of age) were divided into two groups. The mice in one group were given a control diet (1.42% Ca, 1.16% P) and the other a high-calcium and high-phosphate diet (2.00% Ca, 3.00% P) for 30 days. Blood was collected from the mice every fifth day for measurement of the calcium and phosphate concentrations in serum. Both ground and decalcified cross-sections were prepared from incisors from the mandible and maxilla for microscopic examination. The levels of serum Ca and P were almost constant in normal mice, regardless of diet. On the other hand, serum P levels in Hyp mice fed the control diet were significantly lower than those in normal mice. The ten days' feeding of the high-Ca/-P diet significantly elevated the serum P level in Hyp mice, and it reached a level similar to that of the normal mice. However, histopathological examination showed no significant changes in incisor dentin of Hyp mice fed the high-Ca/-P diet, and interglobular dentin still occurred. These results suggest that the multiple formations of interglobular dentin, which is the most outstanding feature of X-linked hypophosphatemic vitamin-D-resistant rickets, are not influenced in Hyp mice by the short-time normalization of the serum phosphate level.