Balbiani ring mRNPs diffuse through and bind to clusters of large intranuclear molecular structures.
Balbiani ring mRNPs diffuse through and bind to clusters of large intranuclear molecular structures.
复制标题
Balbiani 环 mRNP 扩散并结合到大型核内分子结构簇上。
DOI：
10.1016/j.bpj.2010.08.004
复制
发表时间：
2010
影响因子：
3.4
通讯作者：
Kubitscheck,Ulrich
中科院分区：
文献类型：
--
作者：
Veith,Roman;Sorkalla,Thomas;Baumgart,Eugen;Anzt,Johannes;Haberlein,Hanns;Tyagi,Sanjay;Siebrasse,JanPeter;Kubitscheck,Ulrich
A detailed conception of intranuclear messenger ribonucleoprotein particle (mRNP) dynamics is required for the understanding of mRNP processing and gene expression outcome. We used complementary state-of-the-art fluorescence techniques to quantify native mRNP mobility at the single particle level in living salivary gland cell nuclei. Molecular beacons and fluorescent oligonucleotides were used to specifically label BR2.1 mRNPs by an in vivo fluorescence in situ hybridization approach. We characterized two major mobility components of the BR2.1 mRNPs. These components with diffusion coefficients of 0.3 ± 0.02μm2/s and 0.73 ± 0.03μm2/s were observed independently of the staining method and measurement technique used. The mobility analysis of inert tracer molecules revealed that the gland cell nuclei contain large molecular nonchromatin structures, which hinder the mobility of large molecules and particles. The mRNPs are not only hindered by these mobility barriers, but in addition also interact presumably with these structures, what further reduces their mobility and effectively leads to the occurrence of the two diffusion coefficients. In addition, we provide evidence that the remarkably high mobility of the large, 50 nm-sized BR2.1 mRNPs was due to the absence of retarding chromatin.
登录
查看更多内容
影响因子：
3.7
作者：
Dross N;Spriet C;Zwerger M;Müller G;Waldeck W;Langowski J
通讯作者：
Langowski J
真核细胞核中的单分子追踪
影响因子：
4.3
作者：
J. Siebrasse;D. Grünwald;U. Kubitscheck
通讯作者：
U. Kubitscheck
影响因子：
3.4
作者：
Saxton, Michael J.
通讯作者：
Saxton, Michael J.
DOI：
10.1073/pnas.111145498
发表时间：
2001-06-19
影响因子：
11.1
作者：
Daneholt, B
通讯作者：
Daneholt, B
DOI：
10.1073/pnas.95.11.6043
发表时间：
1998-05-26
影响因子：
11.1
作者：
Politz, JC;Browne, ES;Pederson, T
通讯作者：
Pederson, T