Mitochondrial DNA sequence-based population genetic structure of the firefly, Pyrocoelia rufa (Coleoptera: Lampyridae)
Mitochondrial DNA sequence-based population genetic structure of the firefly, Pyrocoelia rufa (Coleoptera: Lampyridae)
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DOI：
10.1023/b:bigi.0000007777.87407.1b
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发表时间：
2003-12-01
影响因子：
2.4
通讯作者：
Jin, BR
中科院分区：
文献类型：
--
作者：
Lee, SC;Bae, JS;Jin, BR
遗传分歧，种群遗传结构，和可能的物种的韩国萤火虫，Pyrocoelia rufa，进行了调查，在韩国中南部大陆，沿海岛屿，一个偏远的近海岛屿，济都岛，和对马岛在日本。从线粒体COI蛋白编码基因的DNA序列分析显示20个mtDNA序列为基础的单倍型，最大分歧为5.5%。系统发育分析采用PAUP、PHYLIP和networks将P. rufa分为两个分支（称为分支A和B），它们之间的最小核苷酸分歧为3.7%。进化枝A分布于韩国大陆、沿海岛屿和日本对马岛，而进化枝B仅分布于济州岛。在种群遗传结构分析中，B支形成了一个独立的地理群，而A支又进一步分为三个群：两个群分别分布在朝鲜半岛的西部和东部，另一个群分布在东部沿海的一个小岛和日本的对马岛。从种群发生和种群结构两方面考虑，济州岛种群与其它种群有明显的区别，而对马岛种群是韩国巨济岛的一个亚群。我们解释了济州岛人口的隔离和对马岛与韩国沿海岛屿的晚更新世-全新世事件的分组。朝鲜大陆的东西分区部分是由于一条大型山脉的存在，该山脉将朝鲜半岛的中部分为西部和东部。
The genetic divergence, population genetic structure, and possible speciation of the Korean firefly, Pyrocoelia rufa, were investigated on the midsouthern Korean mainland, coastal islets, a remote offshore island, Jedu-do, and Tsushima Island in Japan. Analysis of DNA sequences from the mitochondrial COI protein-coding gene revealed 20 mtDNA-sequence-based haplotypes with a maximum divergence of 5.5%. Phylogenetic analyses using PAUP, PHYLIP, and networks subdivided the P. rufa into two clades ( termed clade A and B) and the minimum nucleotide divergence between them was 3.7%. Clade A occurred throughout the Korean mainland and the coastal islets and Tsushima Island in Japan, whereas clade B was exclusively found on Jeju-do Island. In the analysis of the population genetic structure, clade B formed an independent phylogeographic group, but clade A was further subdivided into three groups: two covering western and eastern parts of the Korean peninsula, respectively, and the other occupying one eastern coastal islet and Japanese Tsushima Island. Considering both phylogeny and population structure of P. rufa, the Jeju-do Island population is obviously differentiated from other P. rufa populations, but the Tsushima Island population was a subset of the Korean coastal islet, Geoje. We interpreted the isolation of the Jeju-do population and the grouping of Tsushima Island with Korean coastal islets in terms of Late Pleistocene-Holocene events. The eastern - western subdivision on the Korean mainland was interpreted partially by the presence of a large major mountain range, which bisects the midpart of the Korean peninsula into western and eastern parts.