Real-time analysis of living animals and rapid screening of human fluid samples using remote sampling electrospray ionization mass spectrometry
Real-time analysis of living animals and rapid screening of human fluid samples using remote sampling electrospray ionization mass spectrometry
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DOI：
10.1016/j.jpba.2019.04.050
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发表时间：
2019-08-05
影响因子：
3.4
通讯作者：
Chen, Lee Chuin
中科院分区：
文献类型：
--
作者：
Yoshimura, Kentaro;Yamada, Yuki;Chen, Lee Chuin
利用新型远程采样电喷雾离子化（RS-ESI）探针对活体动物和生物样品进行了实时和原位质谱分析。与传统的ESI不同，在传统的ESI中，进样需要进样或注射器加载，RS-ESI探针在进样毛细管与样品接触时电离样品。由于采样毛细管是电保持在地电位，动物和操作员的安全得到保证。液体样品通过文丘里效应被吸入毛细管另一端的ESI发射器。随后，当高电压施加到放置在离子源腔室内的对电极时，产生电喷雾。探针单元通过一根长软管连接到质谱仪上，在分析过程中可以自由操纵其位置。在这份报告中，我们展示了一个活的小鼠肝脏的实时分析和自动分析138血清样本使用这种新技术。(C)2019爱思唯尔B. V.保留所有权利。
Real-time and in-situ mass-spectrometry analyses of living animal and biological sample were performed using a novel remote sampling electrospray ionization (RS-ESI) probe. Unlike conventional ESI, in which injection or syringe loading is required for sample introduction, the RS-ESI probe ionizes the samples when the sampling capillary is in contact with the sample. As the sampling capillary is electrically held at ground potential, the safety of the animal and operator is assured. The liquid sample is aspirated to the ESI emitter at the other end of the capillary by the Venturi effect. Subsequently, the electrospray is generated when a high voltage is applied to the counter electrode placed inside the ion source chamber. The probe unit is attached to the mass spectrometer with a long flexible tube and its position can be freely manipulated during the analysis. In this report, we demonstrate a real-time analysis of a living mouse liver and an automatic analysis of 138 serum samples using this new technique. (C) 2019 Elsevier B.V. All rights reserved.