The expression of the gastrin/cholecystokinin (GAST/CCK) family and their receptors (CCKAR/CCKBR) in the chicken changes in response to quantitative restriction and reveals a functional role of CCK in the crop
The expression of the gastrin/cholecystokinin (GAST/CCK) family and their receptors (CCKAR/CCKBR) in the chicken changes in response to quantitative restriction and reveals a functional role of CCK in the crop
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DOI：
10.1016/j.ygcen.2022.114024
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发表时间：
2022-03-15
影响因子：
2.7
通讯作者：
Dunn, Ian C.
中科院分区：
文献类型：
--
作者：
Jiao, Yuping;Wilson, Peter W.;Dunn, Ian C.
胃泌素和胆囊收缩素肽结合共同的G蛋白偶联受体胆囊收缩素受体B（CCKBR），而胆囊收缩素受体A（CCKAR）优先与CCK结合。胃泌素和胆囊收缩素介导来自胃肠道的信号以调节食欲和消化功能。在这项研究中，胆囊收缩素/胃泌素家族的表达及其受体表达的分布被测量，以了解肽的靶器官以及表达如何响应食物摄入量的变化。我们证实了胃窦中胃泌素的限制性表达和下丘脑中胆囊收缩素的丰富表达。解除限饲后，肉种鸡胃窦中胃泌素的表达显著升高。CCK BR在下丘脑和前胃中含量最高。CCKAR在胰腺和胰头中含量最高，是胃肠道的十倍以上。取消食物限制后，胰腺中的胆囊收缩素表达增加。胃肠道中的CCKAR在回肠远端周围达到峰值，位于胆囊收缩素表达峰值的远端。盲肠中几乎没有胆囊收缩素的表达，但CCKAR的表达很高。作物中的CCKAR表达是出乎意料的，支持胆囊收缩素在介导作物排空中的作用，这得到了胆囊收缩素给药后体外收缩观察的支持。胆囊收缩素/胃泌素家族及其受体对食物摄入量和表达模式变化的反应将刺激并告知关于其在家禽生长中的作用的新假设。
Gastrin and cholecystokinin peptides bind a common G-protein coupled receptor, cholecystokinin receptor B (CCKBR) whilst cholecystokinin receptor A (CCKAR) is preferentially bound by CCK. Gastrin and cholecystokinin mediate signalling from the gastrointestinal tract to regulate appetite and digestive function. In this study, expression of the cholecystokinin/gastrin family and distribution of their receptors expression was measured to understand the target organs for the peptides and how expression responds to changes in food intake. We confirmed the restricted expression of gastrin in the antrum and the abundant expression of cholecystokinin in the hypothalamus. The expression of gastrin in the antrum was significantly elevated in broiler breeders when released from feed restriction. CCKBR was most abundant in the hypothalamus and proventriculus. CCKAR was most abundant in the pancreas and crop, more than tenfold greater than the gastrointestinal tract. Cholecystokinin expression in the pancreas increased after removal of food restriction. CCKAR in the gastrointestinal tract peaks around the distal ileum, distal to the peak of cholecystokinin expression. There was virtually no cholecystokinin expression in the caecum but CCKAR expression was high. The CCKAR expression in the crop was unexpected, supporting a role of cholecystokinin in mediating crop emptying which was supported by the observation of in-vitro contraction after cholecystokinin administration. The response to changes in food intake and the expression pattern of the cholecystokinin/gastrin family and their receptors will stimulate and inform new hypotheses on their role in growth in poultry.