Regulated tissue fluidity steers zebrafish body elongation
Regulated tissue fluidity steers zebrafish body elongation
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DOI：
10.1242/dev.090381
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发表时间：
2013-02-01
期刊：
影响因子：
4.6
通讯作者：
Holley, Scott A.
中科院分区：
文献类型：
--
作者：
Lawton, Andrew K.;Nandi, Amitabha;Holley, Scott A.
尾芽是脊椎动物胚胎发育过程中的后前缘，由中轴骨骼、肌肉组织和脊髓的能动祖细胞组成。我们测量了斑马鱼尾芽的三维细胞流场，并确定了组织流动性的变化，这些变化是由细胞运动的连贯性降低而不改变细胞速度所揭示的。我们发现了一个有方向的后方流动，其中个别细胞运动之间的极化是高的，反映了有序的集体迁移。在尾芽的后尖端，由于单个细胞运动的方向变化增加，这种流动使得大量细胞混合促进了急剧的双边转弯。抑制Wnt或Fgf信号传导或钙粘蛋白2功能降低了流动的连贯性，但对躯干和尾部延伸具有不同的后果。建模和额外的数据分析表明，细胞流的连贯性和速率之间的平衡决定了身体伸长是否是线性的，或者是否在流动中形成拥塞，身体轴是否扭曲。
The tailbud is the posterior leading edge of the growing vertebrate embryo and consists of motile progenitors of the axial skeleton, musculature and spinal cord. We measure the 3D cell flow field of the zebrafish tailbud and identify changes in tissue fluidity revealed by reductions in the coherence of cell motion without alteration of cell velocities. We find a directed posterior flow wherein the polarization between individual cell motion is high, reflecting ordered collective migration. At the posterior tip of the tailbud, this flow makes sharp bilateral turns facilitated by extensive cell mixing due to increased directional variability of individual cell motions. Inhibition of Wnt or Fgf signaling or cadherin 2 function reduces the coherence of the flow but has different consequences for trunk and tail extension. Modeling and additional data analyses suggest that the balance between the coherence and rate of cell flow determines whether body elongation is linear or whether congestion forms within the flow and the body axis becomes contorted.