Growth suppressor lingerer regulates bantam microRNA to restrict organ size
Growth suppressor lingerer regulates bantam microRNA to restrict organ size
复制标题
生长抑制因子通过调节 bantam microRNA 来限制器官大小。
DOI：
10.1093/jmcb/mjv045
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发表时间：
2015-10-01
影响因子：
5.5
通讯作者：
Zhang, Lei
中科院分区：
文献类型：
--
作者：
Dong, Liang;Li, Jinhui;Zhang, Lei
进化上保守的Hippo信号通路通过调节细胞增殖和凋亡在器官大小控制中起重要作用。在这里，我们使用RNAi修饰筛选在果蝇中鉴定出Lingerer （Lig）是一种生长抑制因子。lig的缺失增加了器官大小，上调了bantam （ban）和Hippo通路靶基因的表达，而lig的过表达导致ban表达减少和器官大小减小。研究表明，Lig - c末端在生长和ban表达中表现出显性负向功能，因此在器官大小控制和ban调控中起重要作用。此外，我们提供的证据表明，Yki和Mad都是光诱导的ban表达所必需的。我们还发现Lig通过Yorkie部分调节Hippo通路靶基因的表达。此外，我们发现光物理相互作用，并要求萨尔瓦多限制细胞生长。综上所述，我们证明了Lig作为一个关键的生长抑制因子，通过ban和Hippo信号来控制器官大小。
The evolutionarily conserved Hippo signaling pathway plays an important role in organ size control by regulating cell proliferation and apoptosis. Here, we identify Lingerer (Lig) as a growth suppressor using RNAi modifying screen in Drosophila melanogaster. Loss of lig increases organ size and upregulates bantam (ban) and the expression of the Hippo pathway target genes, while overexpression of lig results in diminished ban expression and organ size reduction. We demonstrate that Lig C-terminal exhibits dominant-negative function on growth and ban expression, and thus plays an important role in organ size control and ban regulation. In addition, we provide evidence that both Yki and Mad are essential for Lig-induced ban expression. We also show that Lig regulates the expression of the Hippo pathway target genes partially via Yorkie. Moreover, we find that Lig physically interacts with and requires Salvador to restrict cell growth. Taken together, we demonstrate that Lig functions as a critical growth suppressor to control organ size via ban and Hippo signaling.