GENETIC AND MOLECULAR-IDENTIFICATION OF A DROSOPHILA HISTIDINE-DECARBOXYLASE GENE REQUIRED IN PHOTORECEPTOR TRANSMITTER SYNTHESIS
GENETIC AND MOLECULAR-IDENTIFICATION OF A DROSOPHILA HISTIDINE-DECARBOXYLASE GENE REQUIRED IN PHOTORECEPTOR TRANSMITTER SYNTHESIS
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DOI：
10.1002/j.1460-2075.1993.tb05732.x
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发表时间：
1993-03-01
期刊：
影响因子：
11.4
通讯作者：
PAK, WL
中科院分区：
文献类型：
--
作者：
BURG, MG;SARTHY, PV;PAK, WL
在视网膜电图（ERG）开/关瞬时缺陷的单一互补组中，发现果蝇的光感受器递质组胺合成缺陷，并以基因剂量依赖性的方式存在，这表明突变体（hdc）可能是果蝇组氨酸脱羧酶（hdc）的结构基因。从大鼠HDC cDNA中分离出一个果蝇同源序列，该序列在476个氨基酸的区域中与哺乳动物HDC具有约60%的同源性。在RNA印迹中，果蝇同源物检测到四种转录本，这些转录本主要在眼睛中表达，在hdc突变体中严重减少。克隆的果蝇cDNA与染色体46F区杂交，并在P元素介导的种系转化中恢复了hdc突变的表型。结果表明，果蝇的同源基因与hdc突变体鉴定的组氨酸脱羧酶基因相对应，该基因的突变破坏了光感受器突触的传递。
Drosophila mutants of a single complementation group with defective on-/off-transients of the electroretinogram (ERG) were found to be deficient in synthesis of the photoreceptor transmitter, histamine, in a gene-dosage dependent manner, suggesting that the gene identified by the mutants (hdc) might be the structural gene for Drosophila histidine decarboxylase (HDC). A rat HDC cDNA was used to isolate a Drosophila homolog which shows approximately 60% sequence identity with mammalian HDCs over a region of 476 amino acids. In RNA blots, the Drosophila homolog detects four transcripts that are expressed primarily in the eye and are severely reduced in hdc mutants. The cloned Drosophila cDNA hybridizes to the 46F region of the chromosome, to which hdc mutations have been mapped, and rescues the hdc mutant phenotype in transgenic flies generated by P element-mediated germline transformation. The results thus show that the Drosophila homolog corresponds to the histidine decarboxylase gene, identified by the hdc mutants, and that mutations in the gene disrupt photoreceptor synaptic transmission.