Identification of circular RNAs hsa_circ_0044235 and hsa_circ_0068367 as novel biomarkers for systemic lupus erythematosus
Identification of circular RNAs hsa_circ_0044235 and hsa_circ_0068367 as novel biomarkers for systemic lupus erythematosus
复制标题
鉴定环状 RNA hsa_circ_0044235 和 hsa_circ_0068367 作为系统性红斑狼疮的新型生物标志物
DOI：
10.3892/ijmm.2019.4302
复制
发表时间：
2019-10-01
影响因子：
5.4
通讯作者：
Li, Junming
中科院分区：
文献类型：
--
作者：
Luo, Qing;Zhang, Lu;Li, Junming
环状RNA(CircRNAs)是一种新的疾病诊断生物标志物。然而，系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)中CircRNAs的表达谱及其临床意义尚不清楚。本研究采用基因芯片技术检测SLE患者和健康对照(HCS)外周血单个核细胞CircRNAs的表达谱，并用逆转录-定量聚合酶链式反应进行验证。共有1,603个CircRNA在SLE患者的PBMC中显著异常表达。对30例系统性红斑狼疮患者和20例肝细胞癌患者的验证分析表明，系统性红斑狼疮患者的hSA_CIRC_0044235和hSA_CIRC_0068367水平显著降低。受试者工作特征曲线分析提示hSA_CIRC_0044235和hSA_CIRC_0068367对系统性红斑狼疮的诊断有重要意义。此外，在45例系统性红斑狼疮、38例HC和30例类风湿关节炎患者中，hSA_CIRC_0044235和hSA_CIRC_0068367对系统性红斑狼疮的诊断潜力得到了验证。此外，在初发SLE患者和抗双链抗体和抗核糖体蛋白P抗体阳性患者中，SLE患者PBMC中hSA_CIRC_0044235水平显著升高。此外，系统性红斑狼疮患者PBMC中hSA_CIRC_0044235的微RNA(MiRNA)靶标hsa-miRNA-892a水平显著升高。提示CircRNAs表达异常可能在系统性红斑狼疮的发病机制中起一定作用，外周血中hSA_CIRC_0044235和hSA_CIRC_0068367水平可作为系统性红斑狼疮诊断的生物标志物。
Circular RNAs (circRNAs) have emerged as novel biomarkers for disease diagnosis. However, the expression profiles and clinical significance of circRNAs in peripheral blood mononuclear cells (PBMCs) from systemic lupus erythematosus (SLE) remain unclear. In the present study, the expression profile of circRNAs in PBMCs from patients with SLE and healthy controls (HCs) was detected by using microarray analysis and verified by reverse transcription-quantitative polymerase chain reaction. A total of 1,603 circRNAs were identified to be significantly aberrantly expressed in PBMCs from patients with SLE. Validation assays in 30 SLE patients and 20 HCs demonstrated that the levels of hsa_circ_0044235 and hsa_circ_0068367 were significantly decreased in the patients with SLE. Receiver operating characteristic curve analysis suggested that hsa_circ_0044235 and hsa_circ_0068367 were significant for SLE diagnosis. Furthermore, the diagnostic potential of hsa_circ_0044235 and hsa_circ_0068367 for SLE was validated in an independent validation set with 45 patients with SLE, 38 HCs and 30 patients with rheumatoid arthritis. In addition, the level of hsa_circ_0044235 in the PBMCs from patients with SLE were identified to be significantly increased in new-onset SLE patients and in patients who were determined to be positive for anti-double-stranded DNA and anti-ribosomal protein P antibodies. Additionally, the level of a microRNA (miRNA) target of hsa_circ_0044235, hsa-miRNA-892a, was identified to be significantly increased in the PBMCs from patients with SLE. The present study suggested that the dysregulation of circRNAs may serve a role in SLE pathogenesis, and that the levels of hsa_circ_0044235 and hsa_circ_0068367 in PBMC have potential as biomarkers for SLE diagnosis.