Accumulation of Biofilm on Ti–6Al–4V Alloy Fabricated Using Additive Layer Manufacturing
Accumulation of Biofilm on Ti–6Al–4V Alloy Fabricated Using Additive Layer Manufacturing
复制标题
使用增材层制造制造的 Ti-6Al-4V 合金上生物膜的积累
DOI：
10.1007/978-3-030-05861-6_81
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
T. Okabe
中科院分区：
文献类型：
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作者：
M. Koike;T. Horie;R. Mitchell;T. Okabe
随着增材制造技术的引入，已经有一些报告描述了其在制造医疗和牙科设备方面的功效。虽然减少生物膜在临床上很重要，但很少有研究将添加剂制造的器件上的生物膜积累与其他方法制造的合金上的生物膜积累进行比较。比较了增材制造的Ti-6Al-4V ELI与传统牙用失蜡铸造合金（纯钛、Ti-6Al-4V ELI合金、Au-Cu-Ag合金、Pd-Ag-Cu-Au合金、Ag-Sn-Zn合金和Co-Cr合金）上变形链球菌atcc 25175的生物膜积累情况。Teflon®磁盘作为对照。在37℃厌氧条件下，对每种合金（n = 3）进行生物膜积累。4 h后，代谢活性物的数量。用生物发光法测定每一种合金上的变形。虽然添加剂制备的Ti-6Al-4V ELI合金表面比铸造合金表面粗糙，但意想不到的是，这种粗糙度并未增加生物膜的积累。显然，即使表面粗糙，合金成分也能最大限度地减少生物膜的积累。
Following the introduction of additive manufacturing technology, there have been several reports describing its efficacy for fabricating medical and dental devices. Although minimizing biofilms is important clinically, few studies have compared biofilm accumulation on additive manufactured devices with that on alloys fabricated by other methods.Streptococcus mutansATCC 25175 biofilm accumulation on additive manufactured Ti–6Al–4V ELI was compared with that on conventional dental alloys fabricated by lost-wax casting (Commercially pure titanium , Ti–6Al–4V ELI alloy, Au–Cu–Ag alloy, Pd–Ag–Cu–Au alloy, Ag–Sn–Zn alloy, and Co–Cr alloy). Teflon®disks served as controls. Biofilm accumulation on each alloy (n = 3) was conducted at 37 °C under anaerobic conditions. After 4 h, the number of metabolically activeS. mutansadhering to each alloy was determined using a bioluminescence assay . Although the surfaces of the additive manufactured Ti–6Al–4V ELI alloy were rougher than those of cast alloys, this roughness, unexpectedly, did not increase the biofilm accumulation. Apparently, alloy composition minimizes biofilm accumulation even when the surface is rough.