Circadian control of eclosion:: Interaction between a central and peripheral clock in Drosophila melanogaster
Circadian control of eclosion:: Interaction between a central and peripheral clock in Drosophila melanogaster
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DOI：
10.1016/s0960-9822(03)00167-2
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发表时间：
2003-03-18
期刊：
影响因子：
9.2
通讯作者：
Sehgal, A
中科院分区：
文献类型：
--
作者：
Myers, EM;Yu, JJ;Sehgal, A
黑腹果蝇在休息时表现出明显的昼夜节律：活动、行为和羽化。这些节律具有大约24小时的内源性周期，并且可以调节或“夹带”环境输入，例如光。昼夜节律依赖于一个功能分子时钟，包括核心时钟基因周期和永恒（在[1]和[2]中综述）。虽然我们知道大脑外侧神经元（LNs）中的时钟控制着休息：活动节律[3]，但对羽化节律的细胞基础还不太了解。我们表明，LN时钟是不足以驱动羽化节律。我们确定，前胸腺（PG），苍蝇发育所需的组织，包含一个功能时钟在羽化的时候。这个时钟是正常的羽化节奏所必需的。然而，PG时钟功能和羽化节律都需要LN的存在。此外，我们证明，色素分散因子（PDF），从LN分泌的神经肽，是必要的PG时钟和羽化节律。与果蝇周边的其他时钟不同，PG类似于哺乳动物的周边振荡器，因为它依赖于来自中央起搏细胞的输入，包括PDF。这是第一份关于昼夜节律事件所必需的外围时钟的报告。
Drosophila melanogaster display overt circadian rhythms in rest:activity behavior and eclosion. These rhythms have an endogenous period of approximately 24 hr and can adjust or "entrain" to environmental inputs such as light. Circadian rhythms depend upon a functioning molecular clock that includes the core clock genes period and timeless (reviewed in [1] and [2]). Although we know that a clock in the lateral neurons (LNs) of the brain controls rest:activity rhythms [3], the cellular basis of eclosion rhythms is less well understood. We show that the LN clock is insufficient to drive eclosion rhythms. We establish that the prothoracic gland (PG), a tissue required for fly development, contains a functional clock at the time of eclosion. This clock is required for normal eclosion rhythms. However, both the PG clock function and eclosion rhythms require the presence of LNs. In addition, we demonstrate that pigment-dispersing factor (PDF), a neuropeptide secreted from LNs, is necessary for the PG clock and eclosion rhythms. Unlike other clocks in the fly periphery, the PG is similar to mammalian peripheral oscillators because it depends upon input, including PDF, from central pacemaker cells. This is the first report of a peripheral clock necessary for a circadian event.