Stability of poly(ethylene oxide)-modified myoglobin in ion conductive polymers at high temperatures
Stability of poly(ethylene oxide)-modified myoglobin in ion conductive polymers at high temperatures
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DOI：
10.1016/s0167-2738(98)00369-5
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发表时间：
1998-12-01
影响因子：
3.2
通讯作者：
Ohno, H
中科院分区：
文献类型：
--
作者：
Kawahara, NV;Ohno, H
离子导电聚醚低聚物被用作血红素蛋白的电化学氧化还原反应的基质。用可见光谱法研究了聚氧化乙烯修饰肌红蛋白（PEG-Mb）在不同温度下的氧化还原活性和稳定性。合成的PEG-Mb在室温下可溶于平均分子量为200的PEO低聚物（PEO 200）中，并且其在120 ℃下表现出氧化和还原形式的可见光谱，这与在30 ℃下的那些相似。通过改变所施加的电势，在80 ℃下将PEO 200中的PEO-Mb重复还原和氧化至少2.5小时。还在80摄氏度下证明了含水量对PEO 200中PEG-Mb热稳定性的影响。当水含量小于10重量%时，氧化的PEO-Mb在PEO 200中在80 ℃下的吸光度稳定至少6小时。含有13.7重量%和更多的水的PEO低聚物使得PEG-Mb在80 ℃下不稳定。PEO低聚物中的PEO-Mb的热稳定性通过添加超过10重量%的水而显著降低。PEG-Mb的这种极端热稳定性的起源被认为是由于用作溶剂的PEG低聚物。(C)1998年由Elsevier Science B. V.出版，版权所有。
Ion conductive polyether oligomers were used as a matrix for the electrochemical redox reaction of heme proteins. The redox activity and stability of poly(ethylene oxide)modified myoglobin (PEG-Mb) in PEO oligomers were investigated at various temperatures with visible spectroscopy. Synthesized PEG-Mb was soluble in PEO oligomers with average molecular weight of 200 (PEO200) at room temperature, and it exhibited visible spectra for the oxidized and reduced form at 120 degrees C which is similar to those at 30 degrees C. PEO-Mb in PEO200 was reduced and oxidized repeatedly at 80 degrees C for at least 2.5 h by changing the applied potential. The effect of water content on the thermal stability of PEG-Mb in PEO200 was also demonstrated at 80 degrees C. The absorbance of the oxidized PEO-Mb in PEO200 at 80 degrees C was stable for at least 6 h when the water content was less than 10 wt%. The PEO oligomers, containing water of 13.7 wt% and more, made the PEG-Mb unstable at 80 degrees C. The thermal stability of PEO-Mb in PEO oligomers was considerably reduced by the addition of water for more than 10 wt%. The origin of this extreme thermal stability of PEG-Mb was concluded to be due to the PEG oligomers used as a solvent. (C) 1998 Published by Elsevier Science B.V. All rights reserved.