Amino acid sequences of Euglena viridis ferredoxin and cytochromes c.
Amino acid sequences of Euglena viridis ferredoxin and cytochromes c.
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绿眼虫铁氧还蛋白和细胞色素的氨基酸序列c．
DOI：
10.1042/bj2760047
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Meyer,TE
中科院分区：
文献类型：
--
作者：
Ambler,RP;Kamen,MD;Bartsch,RG;Meyer,TE
裸藻目包括许多种，可能有40个属，但几乎所有的生物化学和遗传学研究都局限于单一种。Euglena gracilis，因为它在实验室中容易生长。E. gracilis表明它们与其他真核细胞系有很大的不同。在本论文中，我们报告的三个蛋白质的序列从另一个眼虫，眼虫绿色，使用材料分离的自然水华。线粒体细胞色素c与E.股薄肌，并包含相同的特征。叶绿体细胞色素c6已分歧到更大的程度，并显示只有77%的同一性。E. viridis与蓝细菌和藻类叶绿体的序列相似，序列同一性高达75%。关于肽的纯化、分析和序列测定实验的细节已作为补充公开号50163（32页）保藏在英国图书馆文献供应中心，Boston Spa，Wetherby，West约克郡LS 23 7BQ，U.K.，可根据Biochem.J.（1991）273，5中所述的条件从其获得拷贝。
The Order Euglenida comprises many species and perhaps 40 genera, but almost all biochemical and genetic studies have been limited to a single species. Euglena gracilis, because of its ease of growth in the laboratory. Sequence studies of chloroplast and mitochondrial proteins from E. gracilis show that they have diverged widely from other eukaryotic lines. In the present paper we report the sequences of three proteins from another euglenoid, Euglena viridis, using material isolated from a natural bloom. The mitochondrial cytochrome c shows more than 90% sequence identity with that from E. gracilis, and contains the same characteristic features. The chloroplast cytochrome c6 has diverged to a greater extent and shows only 77% identity. The chloroplast ferredoxin from E. viridis is similar in sequence to those of cyanobacteria and algal chloroplasts, with sequence identities of up to 75%. Details of the purification, analysis and sequence determination experiments on the peptides have been deposited as Supplementary Publication SUP 50163 (32 pages) at the British Library Document Supply Centre, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1991) 273, 5.