Mineral and matrix changes in Brtl/+ teeth provide insights into mineralization mechanisms.
Mineral and matrix changes in Brtl/+ teeth provide insights into mineralization mechanisms.
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DOI：
10.1155/2013/295812
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发表时间：
2013
影响因子：
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通讯作者：
Marini JC
中科院分区：
文献类型：
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作者：
Boskey AL;Verdelis K;Spevak L;Lukashova L;Beniash E;Yang X;Cabral WA;Marini JC
Brt 1/+小鼠是IV型骨生成的敲入模型，其中Gly 349 Cys置换被引入一个COL 1A 1等位基因。为了深入了解这些转基因小鼠牙本质结构和矿物质成分的变化，本研究的目的是使用微型计算机断层扫描（micro-CT）、扫描电子显微镜（SEM）和傅里叶变换红外成像（FTIRI）来分析这些结构在2和6个月大时。结果与IV型OI患者的牙齿表型一致，显示牙齿中的磨牙体积减少和矿化组织体积减少，而牙釉质性质没有变化。通过FTIRI在2个月和6个月时注意到酸性磷酸盐含量增加，并且在Brt 1/+牙齿中在2个月而不是6个月时注意到胶原结构改变的趋势。酸性磷酸盐含量的增加表明矿化过程的延迟，最有可能与胶原结构的缺陷有关。似乎在Brt 1/+牙齿中，矿化结构的缓慢成熟允许校正改变的矿物质含量和酸性磷酸盐分布。
The Brtl/+ mouse is a knock-in model for osteogenesis imperfecta type IV in which a Gly349Cys substitution was introduced into one COL1A1 allele. To gain insight into the changes in dentin structure and mineral composition in these transgenic mice, the objective of this study was to use microcomputed tomography (micro-CT), scanning electron microscopy (SEM), and Fourier transform infrared imaging (FTIRI) to analyze these structures at 2 and 6 months of age. Results, consistent with the dental phenotype in humans with type IV OI, showed decreased molar volume and reduced mineralized tissue volume in the teeth without changes in enamel properties. Increased acid phosphate content was noted at 2 and 6 months by FTIRI, and a trend towards altered collagen structure was noted at 2 but not 6 months in the Brtl/+ teeth. The increase in acid phosphate content suggests a delay in the mineralization process, most likely associated with the defect in the collagen structure. It appears that in the Brtl/+ teeth slow maturation of the mineralized structures allows correction of altered mineral content and acid phosphate distribution.