Purification, composition analysis and antioxidant activity of the polysaccharides from Dendrobium nobile Lindl.
Purification, composition analysis and antioxidant activity of the polysaccharides from Dendrobium nobile Lindl.
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DOI：
10.1016/j.carbpol.2009.10.033
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发表时间：
2010-03-17
影响因子：
11.2
通讯作者：
Chun, Ze
中科院分区：
文献类型：
--
作者：
Luo, AoXue;He, XingJin;Chun, Ze
从石斛茎中提取粗水溶性多糖。用沸水。采用DEAE-cellulose和Sephadex G-200色谱柱对多糖进行纯化，得到DNP1-1、DNP2-1、DNP3-1、dnp2 -2 4个主要多糖组分。凝胶渗透色谱（GPC）分析表明，DNP1-1、DNP2-1、DNP3-1、dnp2 -2的平均分子量（Mw）分别约为136 kDa、27.7 kDa、11.8 kDa和11.4 kDa。单糖分析表明，甘露糖、葡萄糖、半乳糖占主导地位，鼠李糖、阿拉伯糖和木糖含量较少。抗氧化活性评价表明，DNP4-2对ABTS自由基具有良好的清除活性，对羟基自由基和DPPH自由基的清除活性高于其他多糖，应作为一种新的潜在抗氧化剂加以开发。(c) 2009 Elsevier Ltd.版权所有。
Crude water-soluble polysaccharides were extracted from the stem of Dendrobium nobile Lindl. using boiling-water. The polysaccharides were successively purified by chromatography on DEAE-cellulose and Sephadex G-200 column, giving four major polysaccharide fractions termed DNP1-1, DNP2-1, DNP3-1, DNP4-2. The gel permeation chromatography (GPC) analysis showed that the average molecular weight (Mw) of polysaccharides (DNP1-1, DNP2-1, DNP3-1, DNP4-2) were approximately 136 kDa, 27.7 kDa, 11.8 kDa and 11.4 kDa, respectively. Monosaccharides analysis revealed the dominance of mannose, glucose, galactose, and smaller amounts of rhamnose, arabinose and xylose in the four polysaccharides. The evaluation of antioxidant activity suggested that DNP4-2 had good potential for scavenging activity of ABTS radical, and higher scavenging activity of hydroxyl radical and DPPH radical than the other polysaccharides, and should be explored as a novel potential antioxidant. (c) 2009 Elsevier Ltd. All rights reserved.