Volumetric, Nanoscale Optical Imaging of Mouse and Human Kidney via Expansion Microscopy.
Volumetric, Nanoscale Optical Imaging of Mouse and Human Kidney via Expansion Microscopy.
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DOI：
10.1038/s41598-018-28694-2
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发表时间：
2018-07-10
影响因子：
4.6
通讯作者：
Vaughan JC
中科院分区：
文献类型：
--
作者：
Chozinski TJ;Mao C;Halpern AR;Pippin JW;Shankland SJ;Alpers CE;Najafian B;Vaughan JC
尽管光学显微镜是评估肾脏生理学和病理学的有力工具，但传统上它无法分辨小于可见光衍射极限~250 nm的结构。在这里，我们报告了最近开发的超分辨率荧光显微镜方法的优化，验证和应用，称为扩展显微镜（ExM），用于小鼠和人类肾脏组织的体积询问，具有70-75 nm的横向和~250 nm的轴向空间分辨率。使用ExM与标准的共聚焦显微镜，我们解决传统上需要可视化的结构，包括足细胞足突，肾小球基底膜和细胞骨架的细节。这种廉价且易于获得的体积纳米级成像方法能够可视化肾脏组织的精细结构细节，这些细节以前难以或不可能通过传统方法测量。
Although light microscopy is a powerful tool for the assessment of kidney physiology and pathology, it has traditionally been unable to resolve structures separated by less than the ~250 nm diffraction limit of visible light. Here, we report on the optimization, validation, and application of a recently developed super-resolution fluorescence microscopy method, called expansion microscopy (ExM), for volumetric interrogation of mouse and human kidney tissue with 70–75 nm lateral and ~250 nm axial spatial resolution. Using ExM with a standard confocal microscope, we resolve fine details of structures that have traditionally required visualization by electron microscopy, including podocyte foot processes, the glomerular basement membrane, and the cytoskeleton. This inexpensive and accessible approach to volumetric, nanoscale imaging enables visualization of fine structural details of kidney tissues that were previously difficult or impossible to measure by conventional methodologies.
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影响因子：
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作者：
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DOI：
10.1097/01.asn.0000115334.65095.9b
发表时间：
2004-03-01
影响因子：
13.6
作者：
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