RESTRICTED REPLICATION OF ADENOVIRUS TYPE-2 IN MOUSE BALB/3T3 CELLS
RESTRICTED REPLICATION OF ADENOVIRUS TYPE-2 IN MOUSE BALB/3T3 CELLS
复制标题
DOI：
10.1007/bf01315303
复制
发表时间：
1986-01-01
影响因子：
2.7
通讯作者：
STROHL, WA
中科院分区：
文献类型：
--
作者：
SILVERSTEIN, G;STROHL, WA
对腺病毒2型(AD2)体外感染小鼠B3T3细胞的性质进行了研究。在感染吸附MOI为225的病毒后，90%以上的细胞合成了早期和晚期病毒特异性抗原。相比之下，子代病毒的产量只有2倍。104到2倍。106FFU/2倍。105个细胞。产量的范围部分地与从最初的亚克隆开始的细胞世代数有关，产量随着传代水平的提高而增加。感染中心分析表明，只有不到0.5%的感染细胞合成了后代病毒。对感染增殖的B3T3细胞的DNA合成分析表明，细胞DNA合成在病毒DNA合成开始的12小时就开始停止。病毒DNA合成的最大速率大约是受感染的人类细胞的12%。与感染的增殖细胞相比，感染静止的B3T3细胞培养物导致了细胞的诱导以及病毒的DNA合成。晚期基因表达分析检测到大多数病毒多肽的合成，但与受感染的人类细胞相比，多肽II、III、IV和IX的合成速度降低了90%以上。
The nature of the infection of mouse B3T3 cells by adenovirus type 2 (Ad2) has been studied in vitro. Following infection with an adsorbed MOI of 225, more than 90 percent of the cells synthesized both early and late virus-specific antigens. In contrast, the yield of progeny virus varied from only 2 .times. 104 to 2 .times. 106 FFU/2 .times. 105 cells. The range in yields was related, in part, to the number of cell generations from the time of the initial subcloning, the yield increasing with passage level. Infectious center analysis suggested that fewer than 0.5 percent of infected cells synthesized progeny virus. Analysis of DNA synthesis in infected multiplying B3T3 cells demonstrated that cellular DNA synthesis began to be shut off at 12 hours p.i., a time when viral DNA synthesis was beginning. The maximum rate of viral DNA synthesis was approximately 12 percent of that in infected human cells. In contrast to infected multiplying cells, infection of quiescent B3T3 cell cultures resulted in the induction of cellular, along with viral, DNA synthesis. Analysis of late gene expression detected synthesis of most viral polypeptides, but revealed greater than 90 percent reductions in the rate of synthesis of polypeptides II, III, IV, and IX, as compared with infected human cells.