Non-invasive measurement of real-time oxygen flux in plant systems with a self-referencing optrode
Non-invasive measurement of real-time oxygen flux in plant systems with a self-referencing optrode
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DOI：
10.1038/protex.2011.266
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发表时间：
2011-11
期刊：
影响因子：
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通讯作者：
Jinxing Lin;Yinglang Wan;E. McLamore;Lusheng Fan;Huaiqing Hao;D. Porterfield;Zengkai Zhang;Wenjun Wang;Yue Xu
中科院分区：
文献类型：
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作者：
Jinxing Lin;Yinglang Wan;E. McLamore;Lusheng Fan;Huaiqing Hao;D. Porterfield;Zengkai Zhang;Wenjun Wang;Yue Xu
该方案描述了非侵入性微量测试技术和氧光极（NMT-OO）的集成，以量化拟南芥根际氧通量。该光极在细胞水平上测量氧浓度和通量时具有高灵敏度和选择性。特别是，使用自参考方法的NMT-OO的应用避免了环境电磁噪声和滞后/校准漂移，为测量生物物理传输提供了极高的信噪比。我们成功地应用这项技术来测量根际氧通量和代谢在完整的根拟南芥植物。我们描述的系统是一种简单可靠的方法来测量植物中的氧通量，并在细胞学研究中有其他广泛的应用。
This protocol describes an integration of the Non-invasive Micro-test Technique and Oxygen Optrode (NMT-OO) to quantify rhizosphere oxygen fluxes in Arabidopsis. The optrode has high sensitivity and selectivity in the measurement of oxygen concentrations and fluxes at the cellular level. In particular, application of the NMT-OO using the self-referencing method avoids environmental electromagnetic noise and hysteresis/calibration drift, providing extremely high signal-to-noise ratios for measuring biophysical transport. We successfully applied this technique to measure rhizosphere oxygen fluxes and metabolism in intact roots of Arabidopsis plants. The system we describe here is a simple and reliable method for measuring oxygen fluxes in plants and has other broad applications in cytological studies.