Rapid detection of pesticides in honey by solid-phase micro-extraction coupled with electrospray ionization mass spectrometry
Rapid detection of pesticides in honey by solid-phase micro-extraction coupled with electrospray ionization mass spectrometry
复制标题
固相微萃取-电喷雾电离质谱快速检测蜂蜜中的农药
DOI：
10.1002/jms.4380
复制
发表时间：
2020
影响因子：
2.3
通讯作者：
Yao Zhong-Ping
中科院分区：
文献类型：
--
作者：
Choi Yi-Ching;Ng Tsz-Tsun;Hu Bin;Li Rong;Yao Zhong-Ping
食品样品中农药残留的检测对于保障食品质量和安全具有重要意义。检测食品样品中农药的传统方法通常涉及劳动密集型且耗时的样品预处理和色谱分离。本研究采用固相微萃取纤维对基质复杂的大众农产品中的农药进行快速提取和富集，然后直接与电喷雾电离质谱联用进行定性和定量分析。使用该技术研究了莠去津、苯霜灵和抗蚜威三种农药，并评估了它们的分析性能。三种农药的检出限和定量限均符合国际标准的临界值。线性校准曲线具有良好的 R2 系数，所有农药的准确度和精密度均在可接受的范围内。分析时间大大缩短，只需最少的样品制备，且不涉及色谱分离。该技术简单且易于设置，并且可以扩展到其他分析物和样品系统的分析。
Detection of pesticide residues in food samples is important for safeguarding food quality and safety. Conventional approaches for detection of pesticides in food samples typically involve labour-intensive and time-consuming sample pretreatment and chromatographic separation. In this study, solid phase micro-extraction fibres were used to rapidly extract and enrich pesticides in honey, a popular agricultural product with complex matrix, and then directly coupled with electrospray ionization mass spectrometry for qualitative and quantitative analysis. Three pesticides, ie, atrazine, benalaxyl, and pirimicarb, were investigated using the technique and their analytical performances were evaluated. The limits of detection and limits of quantitation of all the three pesticides could fulfil the cut-off values of the international standard. Linear calibration curves were constructed with good R2 coefficients, and the accuracy and precision were in acceptable ranges for all the pesticides. The analysis time is much reduced, with only minimum sample preparation and no chromatographic separation involved. The technique is simple and easy to set up, and can be extended for analysis of other analytes and sample systems.