X-ray-activated persistent luminescence nanomaterials for NIR-II imaging
X-ray-activated persistent luminescence nanomaterials for NIR-II imaging
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DOI：
10.1038/s41565-021-00922-3
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发表时间：
2021-06-10
影响因子：
38.3
通讯作者：
Zhang, Fan
中科院分区：
文献类型：
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作者：
Pei, Peng;Chen, Ying;Zhang, Fan
持续发光不受背景自发荧光的影响，因此有望实现高对比度生物成像。然而，目前，用于体内成像的持久发光材料主要是块体晶体，其特征在于不均匀的尺寸和形态、不可接近的核-壳结构和短发射波长。在这里，我们报告了一系列的X射线激活，镧系元素掺杂的纳米粒子在第二近红外窗口（NIR-II，1000 - 1700 nm）的发射寿命延长。核-壳工程实现了可调谐的NIR-II持续发光，其在信噪比和体内多路复用编码和多级加密的准确性方面优于NIR-II荧光，以及在深层组织（类似于2-4 mm）中解析小鼠腹部血管、肿瘤和输尿管方面优于NIR-II荧光，具有高达四倍的信噪比和三倍的锐度。这些合理设计的纳米颗粒还允许内脏的高对比度多重成像和小鼠肿瘤的多模式NIR-II持续发光-磁共振-正电子发射断层扫描成像。
Persistent luminescence is not affected by background autofluorescence, and thus holds the promise of high-contrast bioimaging. However, at present, persistent luminescent materials for in vivo imaging are mainly bulk crystals characterized by a non-uniform size and morphology, inaccessible core-shell structures and short emission wavelengths. Here we report a series of X-ray-activated, lanthanide-doped nanoparticles with an extended emission lifetime in the second near-infrared window (NIR-II, 1,000-1,700 nm). Core-shell engineering enables a tunable NIR-II persistent luminescence, which outperforms NIR-II fluorescence in signal-to-noise ratios and the accuracy of in vivo multiplexed encoding and multilevel encryption, as well as in resolving mouse abdominal vessels, tumours and ureters in deep tissue (similar to 2-4 mm), with up to fourfold higher signal-to-noise ratios and a threefold greater sharpness. These rationally designed nanoparticles also allow the high-contrast multiplexed imaging of viscera and multimodal NIR-II persistent luminescence-magnetic resonance-positron emission tomography imaging of murine tumours.