Myosin regulation in the migration of tumor cells and leukocytes within a three-dimensional collagen matrix
Myosin regulation in the migration of tumor cells and leukocytes within a three-dimensional collagen matrix
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DOI：
10.1007/s00018-004-4391-6
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发表时间：
2005-01-01
影响因子：
8
通讯作者：
Entschladen, F
中科院分区：
文献类型：
--
作者：
Bastian, P;Lang, K;Entschladen, F
细胞的迁移是一个复杂的调节过程，通过细胞骨架的重组产生动力。在这里，我们对肌球蛋白的表达和参与调节白细胞的生理迁移和肿瘤细胞的病理迁移进行比较研究。我们发现肌球蛋白在迁移中的参与在这两种细胞类型中是不同的。在白细胞中，非肌肉肌球蛋白 II 的活性对于自发（基质诱导）迁移和 G 蛋白偶联受体（即趋化因子和神经递质）配体诱导的迁移至关重要。相反，自发性肿瘤细胞迁移在很大程度上独立于非肌肉肌球蛋白 II 活性，而去甲肾上腺素诱导的迁移则完全被非肌肉肌球蛋白 II 或磷酸化肌球蛋白轻链激酶（即 ROCK 或钙/钙调蛋白依赖性肌球蛋白轻链激酶）的直接抑制所抑制。
The migration of cells is a complex regulatory process which results in the generation of motor forces through the reorganization of the cytoskeleton. Here we present a comparative study of the expression and involvement of myosin in the regulation of the physiological migration of leukocytes and the pathological migration of tumor cells. We show that the involvement of myosin in the migration is distinct in these two cell types. In leukocytes, the activity of non-muscle myosin II is essential for both the spontaneous (matrix-induced) migration and the migration induced by ligands to G protein-coupled receptors, i.e. chemokines and neurotransmitters. In contrast, spontaneous tumor cell migration is largely independent of non-muscle myosin II activity, whereas the norepinephrine-induced migration is completely inhibited by either direct inhibition of non-muscle myosin II or of the kinases phosphorylating the myosin light chain, namely ROCK or the calcium/calmodulin-dependent myosin light-chain kinase.