NERVE GROWTH FACTOR-INDUCED ALTERATION IN THE RESPONSE OF PC12 PHEOCHROMOCYTOMA CELLS TO EPIDERMAL GROWTH-FACTOR
NERVE GROWTH FACTOR-INDUCED ALTERATION IN THE RESPONSE OF PC12 PHEOCHROMOCYTOMA CELLS TO EPIDERMAL GROWTH-FACTOR
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DOI：
10.1083/jcb.88.1.189
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发表时间：
1981-01-01
影响因子：
7.8
通讯作者：
GUROFF, G
中科院分区：
文献类型：
--
作者：
HUFF, K;END, D;GUROFF, G
PC12[大鼠嗜铬细胞瘤]细胞在神经生长因子[NGF]的作用下，在形态和生化上分化为交感神经元样细胞，对表皮生长因子[EGF]有反应。对EGF的反应在某些方面与对NGF的反应相似。这两种肽均能迅速增加细胞粘附和2-脱氧葡萄糖摄取，并诱导鸟氨酸脱羧酶。NGF导致细胞增殖减少，细胞明显肥大。EGF促进细胞增殖，但不引起细胞肥大。NGF诱导神经突的形成；EGF则不然。当这两种因素同时存在时，细胞形成神经突。对EGF的生物反应，如鸟氨酸脱羧酶的诱导，被NGF事先处理的细胞减弱。PC12细胞有EGF受体。EGF与这些受体的结合是快速和特异性的，其平衡常数为1.9倍。每个细胞约有80,000个受体；这个数与细胞密度无关。用NGF处理细胞可使EGF的结合量减少至少80%。受体结合减少在。apprx后开始。NGF治疗12-18 h， 3天内完成。Scatchard图显示结合位点的数量减少，而不是结合位点对EGF的亲和力减少。
PC12 [rat pheochromocytoma] cells, which differentiate morphologically and biochemically into sympathetic neuronlike cells in response to nerve growth factor [NGF] respond to epidermal growth factor [EGF]. The response to EGF is similar in certain respects to the response to NGF. Both peptides produce rapid increases in cellular adhesion and 2-deoxyglucose uptake and induce ornithine decarboxylase. NGF causes a decreased cell proliferation and a marked hypertrophy of the cells. EGF enhances cell proliferation and does not cause hypertrophy. NGF induces the formation of neurites; EGF does not. When both factors are presented simultaneously, the cells form neurites. The biological response to EGF, as exemplified by the induction of ornithine decarboxylase, is attenuated by prior treatment of the cells with NGF. PC12 cells have EGF receptors. The binding of EGF to these receptors is rapid and specific and exhibits an equilibrium constant of 1.9 .times. 10-9 M. Approximately 80,000 receptors are present per cell; this number is independent of cell density. Treatment of the cells with NGF reduces the amount of EGF binding by at least 80%. The decrease in receptor binding begins after .apprx. 12-18 h of NGF treatment and is complete within 3 days. Scatchard plots indicate that the number of binding sites decreases, not the affinity of the binding sites for EGF.